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Fig. 3 | BMC Microbiology

Fig. 3

From: E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells

Fig. 3

Isotype analysis and T helper cell responses. a-c Graphs show the reciprocal endpoint titers of anti-V3 isotypes from mice (n = 5) intranasally immunized with Env(V3)-E2 + CT or E2wt. a IgG1 and b IgG2a serum anti-V3 antibodies. Naïve mouse group (N.I., n = 5) was used as control of background response. c Anti-V3 IgG1 (gray bars) and IgG2a (white bars) endpoint titers for each animal from Env(V3)-E2 plus CT group. Lines represent median values; statistical significance was determined using the unpaired two-tailed Student's t-test; P values are reported. d Ex vivo production of IL-4 and e IFN-γ by purified CD4+ T cells isolated from mice immunized as above, in response to spleen cells pulsed with no antigen (no ag), E2wt, or Env(V3)-E2. Cytokines were detected by ELISA assay; statistical significance was determined using the unpaired two-tailed Student's t-test; P values are reported. A representative experiment out of two is shown

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