Fig. 2

Systemic antibody responses. BALB/c mice (n = 5) were intranasally immunized with Env(V3)-E2 plus CT adjuvant or E2wt particles. At the end of vaccination, sera were collected to determine binding antibody responses against (a) E2wt (white bars), Env(V3)-E2 protein (gray bars) and (b-c) HIV-1 SF162 V3 synthetic peptide, by ELISA. Results were expressed as reciprocal endpoint titers. Naïve mouse group (N.I., n = 5) was used as control of background response. a Anti-carrier E2 and anti- Env-E2 IgG antibodies. Mean values + S.D. are shown. b Anti-V3 IgG and c anti-V3 serum IgA binding antibodies obtained from sera of each animal. Lines represent median values. P values determined using the unpaired two-tailed Student's t-test are reported. A representative experiment out of two is shown