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Fig. 1 | BMC Microbiology

Fig. 1

From: E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells

Fig. 1

Mucosal antibody responses. BALB/c mice (n = 5) were intranasally immunized with: Env(V3)-E2 particles administered with (triangle) or without (square) CT adjuvant. At weekly intervals fecal pellets were collected to determine the presence of E2-binding antibodies and Env-specific IgA. Naïve mouse group (N.I., n = 5, diamonds) was used as control of background response. Fecal anti-E2 and anti-V3 IgA antibodies were measured by coating in ELISA assay (a) E2wt protein or (b) synthetic HIV-1 SF162 V3 peptide (filled triangles or squares), and scrambled peptide (empty triangles or squares). A representative experiment out of two is shown. Graphs show the mean absorbance values (± S.D.) of fecal samples from mice of each group at the indicated time points; statistical significance was determined using the unpaired two-tailed Student's t-test, P value is reported. (c) Specific levels of IgA antibodies to V3 peptide as defined by ELISA OD450 readings normalized to whole IgA concentration in soluble fecal extracts from mice immunized with Env(V3)-E2 particles administered with (triangle) or without (square) CT adjuvant

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BMC Microbiology

ISSN: 1471-2180

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