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Fig. 1 | BMC Microbiology

Fig. 1

From: RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions

Fig. 1

RsmW is upregulated under biofilm growth in P. aeruginosa. a RNA sequencing read mapping showing the genome region and levels of the predicted small RNA (RsmW) during biofilm (BF) and planktonic (P) growth states and from libraries prepared conventionally (LARGE Library) and adapted for small RNA retention (SMALL Library). RPKM (Reads Per Kilobase of transcript per Million mapped reads) were calculated for each small RNA to compare levels between conditions. RsmW was upregulated approximately 21- and 10-fold in biofilm versus planktonic conditions based on RNA-seq and qRT-PCR, respectively (bottom panel). RsmW was categorized as the 3’-untranslated region (3’-UTR) of gene PA4570 because it was less than 30 nucleotides apart from this open reading frame. b Northern blot analyses reveal two bands for RsmW. RNA (10 μg) from P. aeruginosa grown under planktonic or drip-flow biofilm conditions was run on denaturing glyoxal agarose gel (top panels), blotted, probed with radiolabeled riboprobes complementary to RsmW, and exposed for 1 hour (bottom panels). Hybridization bands of approximately 120 bases in planktonic and biofilm growth conditions and an additional band of approximately 400 bases in biofilm conditions are revealed. Lanes: MW, molecular weight ladder in number of bases; 1.) planktonic conditions; 2.) drip-flow biofilm conditions. Abbreviations: BF1, BF2: Biofilm replicates; P1, P2: Planktonic replicates; SEM: standard error of the mean

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