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Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles

BMC Microbiology volume 16, Article number: 144 (2016) Cite this article

Abstract

Background

Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.

Methods

Copper nanoparticles (CuNPs) were prepared biologically using the headspace gases generated by the bacterial culture Escherichia coli Z1. The antimicrobial activity of CuNPs was evaluated against the bacterial strains Bacillus subtilis, Micrococcus luteus, Streptomyces parvulus, Escherichia coli, Pseudomonas aeruginosa as well as some fungal strains Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani and Alternaria solani.

Results

Biological CuNPs demonstrated antibacterial and antifungal activities higher than those of the untreated copper sulfate. At the same time, limestone and sandstone blocks treated with consolidation polymers functionalized with CuNPs recorded apparent antimicrobial activity against E. coli, S. parvulus and B. subtilis in addition to an improvement in the physical and mechanical characters of the treated stones. Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.

Conclusion

Consolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

Background

Nanoparticles demonstrate vast array of properties such as optical, electrical, catalytic, magnetic and biological activities which are diverged from those of the original constituents [1, 2]. The emergence of nanotechnology in the last decade offers occasions for exploring the antimicrobial effect of metal nanoparticles. Some of the biological properties of nanoparticles of various metals have been explored by assaying their antimicrobial susceptibilities. It has been reported that nanoparticles of Ag, Zn, Cu and Au exhibit a wide spectrum of antimicrobial activity against different bacterial [3–5] and fungal species [6–9].

Copper nanoparticles were reported to have antimicrobial activity against wide spectrum of bacteria including Micrococcus luteus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, [10–12]. Moreover, CuNPs have been shown to suppress vegetative growth of some fungal species such as Aspergillus flavus, Aspergillus niger, Alternaria alternata, Fusarium solani, Penicillium chrysogenum and Candida albicans [13, 14].

Elevated levels of heavy metals represent a potential stimulus for metal tolerant bacteria that regularly possess specific metal resistance mechanisms. One of these mechanisms is the intracellular or extracellular transformation of metal ions into insoluble metal particles [15, 16]. The intracellular approach consists of transporting metal ions into the bacterial cell where they are transformed into nanoparticles while the extracellular process involves the trapping of metal ions on the cell surface as metal nanoparticles [17, 18]. Moreover, bacteria can release certain metabolites into their microenvironment that can transform metal ions into less soluble metal particles [19]. Synthesis of metal nanoparticles through bacteria is supported by the fact that the generated particles are environmentally safe and have elevated chemical reactivity [20].

Microorganisms can initiate and accelerate some geochemical reactions leading to biodeterioration of historic monuments [21]. The biodeterioration of archeological stones occurs as a consequence of the intrusion of microorganisms into the components of the mineral lattice [22]. The capability of microbial cells to inhabit stone surface was attributed to numerous aspects such as mineral composition, surface texture, moisture content, pH and nutrient accessibility [23]. In order to protect the archeological artifacts against microorganisms, different inorganic materials such as titanium dioxide and Ag-doped titanium dioxide have been used as antifouling agent by dispersing them in consolidation polymers. [24, 25]. Thus the aim of the present study was to investigate the antimicrobial potentiality of CuNPs synthesized biologically by the bacterial strain Escherichia coli Z1 and its application for the fortification of archaeological stones against microbial inhabitation.

Methods

Consolidation polymers and stone samples

In this study, two consolidation polymers were utilized. The first is Primal AC33 polymer (AC; Dow Chemical Co., USA) which comprises of methylacrylate and ethylmethacrylate. The other one is silicon polymer (S; Wacker BS 1001, Wacker Chemei AG, Germany) that is consisting of silane/siloxane emulsion. Sandstone and limestone samples were used in this study. The physical and mechanical properties of the tested stones including bulk density, water absorption, porosity, compressive strength and tensile strength were characterized before and after treating them with the functionalized polymers according to Essa and Khallaf [19].

Preparation of the Cu-particles

A stock solution of copper sulfate was prepared by dissolving 200 mg of CuSO4 in 200 mL deionized distilled H2O. Different concentrations of CuSO4 solutions (50, 100, 150, 200, 250 μg/mL) were prepared from stock solution. One hundred milliliter of each concentration was exposed to the culture biogases of the bacterial strain Escherichia coli Z1 [26] for 60 min in aerobic bioreactor at 30 °C as described by Essa et al. [27]. Bacterial growth was monitored by measuring the optical density at 600 nm. The produced colloidal solution of each concentration was subjected for ultra-speed centrifugation at 100,000 rpm for 30 min. The collected Cu-particles was suspended in 10 mL deionized distilled H2O and re-centrifuged at 100,000 rpm for 30 min. This step was repeated three times and the collected Cu-particles were suspended in 1 mL dd H2O to assay the antimicrobial activities. Another set of the Cu-particles was suspended in the consolidation polymers at 150 μg/mL for stone treatments.

Antibacterial activity of the Cu-particles

The antibacterial activity of copper particles was assayed against Bacillus subtilis, Micrococcus luteus, Streptomyces parvulus, Escherichia coli Z1 and Pseudomonas aeruginosa. Twenty five milliliter of nutrient broth containing various doses of Cu-particles (50, 100, 150, 200 and 250 μg/mL) were inoculated with 1 mL of a fresh culture of each bacterial strain (O.D = 0.6). After incubation for 48 h at 30 °C, the bacterial growth was monitored spectrophotometrically by measuring the optical density at 600 nm. At the same time, the antibacterial properties of the Cu-particles and CuSO4 were measured using the modified agar well diffusion method of Perez et al., [28]. Nutrient agar plates were inoculated with the different bacterial strains. Once the agar was solidified, it was punched with 8 mm diameter wells and filled with 25 μL of 100 μL/mL CuSO4 and Cu-particles. The experiment was repeated three times with three replicates for each treatment and diameters of the inhibition zones were measured after 24 h incubation at 30 °C. Streptomycin (1000 μg/mL) was used as a positive control.

Antifungal activity of the Cu-particles

The activity of Cu-particles and CuSO4 was measured against Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani and Alternaria solani. These strains were provided by the City of Science & Technology, Egypt. Each fungal strain was grown on potato dextrose agar (PDA) slant and incubated at 25 ± 2 °C for 5 days. Three milliliter sterile distilled water was added to each fungal slant and the fungal spore concentration was determined by haemocytometer. One hundred milliliter PDA containing various Cu-particles or CuSO4 levels (50, 100, 150, 200 and 250 μg/mL) was inoculated with the fungal spore suspensions (106 spore/mL). After incubation at 25 ± 2 °C for 5 days the cultures were filtered through pre-weighed Whatman No.1 filter paper and the filter paper with fungal biomass was dried at 70 °C until constant weight. At the same time antifungal activity of Cu-particles and CuSO4 was evaluated using fungal growth inhibition assay as described by Fiori et al., [29] with some modification. The Cu-particles and CuSO4 were mixed with molten PDA to provide desired concentration (200 μL/mL) and 8 mm diameter disc of each fungal strain was added to the center of PDA plates. After incubation at 25 ± 2 °C for 72 h, colony diameter was measured. Nystatin (300 μg/mL) was used as a positive control.

Treatment of stone blocks with Cu-particles based on polymers

Cu-particles were combined with the consolidation polymers at the concentration 150 μg/mL. The functionalized polymers were used to coat the external surfaces of stone blocks and were left 7 days at room temperature for complete drying.

Antimicrobial activity of the treated stones

The antibacterial activity of the treated stones was assayed according to Essa and Khallaf [19]. One surface of the coated stones was submerged in the bacterial culture (1.0 × 106 cell/mL) for 2 h then they were incubated at 30 °C for 24 h. After that the treated stones were dipped into 10 mL 0.85% NaCl solution for 1 h. One milliliter of the washing solution was diluted 100 times and 0.1 mL of diluted solutions was plated on NA. After incubation at 30 °C for 24 h the bacterial colonies were counted. Untreated stone samples were used as reference. The experiment was repeated three times with three replicates for each treatment.

SEM and EDX of the composite Cu-particles based on polymers

The coated surfaces of the stones were analyzed using scanning electron microscope (JEOL JSM-5410, Japan) meanwhile the chemical analysis of the treated polymers were studied using Electron Dispersive X-ray system connected with the scanning electron microscope.

Statistical methods

The resulted data were tested by using the ANOVA test for significance. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01. All statistical tests were carried out using SPSS (v. 16.0) software.

Results

Antimicrobial activities of the Cu-particles

As a result of pumping the biogenic volatiles of the bacterial strain Escherichia coli Z1 in the copper sulfate solution for short exposure time (60 min), a light blue colloidal solution of copper was obtained. The antibacterial potentiality of the collected Cu-particles was demonstrated at different concentrations. Results in Fig. 1a showed a suppression of the bacterial growth at various levels depending on Cu-particles concentrations. There was no bacterial growth at the concentration 150 μg/mL or above while at 100 μg/mL the percentage of growth reduction reached 94.7 % for E. coli, 92.4 % for M. luteus, 90.9 % for S. parvulus, and 95.5 % for B. subtilis. At the same time, P. aeruginosa demonstrated a clear tolerance against high concentrations of Cu-particles where the percentage growth inhibition was 69.6 % at 150 μg/mL and 79.5 % at 200 μg/mL. In order to confirm the antibacterial activity of Cu-particles in comparison with untreated copper sulfate, another experiment was conducted where the diameter of the inhibition zones was measured. The obtained results (Table 1 and Fig. 2) showed an increase of the antibacterial activity of Cu-particles compared to CuSO4. The maximum inhibition zones were recorded with Cu-particles (150 μg/mL) against E. coli Z1 (38 mm) and S. parvulus (33 mm) while the lowest value was recorded with P. aeruginosa (16 mm).

Fig. 1
figure 1

Antimicrobial activity of different concentrations of CuNPs against bacteria (a) and fungi (b). The bacterial growth was monitored as culture optical density (OD600) while the fungal growth was assayed as biomass dry weight. Data are the means of five replication ± standard errors. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01

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Table 1 Antimicrobial activity of Cu-particles and CuSO4
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Fig. 2
figure 2

Antimicrobial activity of the different copper forms against (a) Escherichia coli Z1, (b) Streptomyces parvulus and (c) Aspergillus flavus where (I) refers to CuSO4 and (II) refers to CuNPs. Antibacterial activity of Cu-particles and CuSO4 was carried out at the concentration 100 μg/mL while antifungal activity was assayed at the concentration 200 μg/mL

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Regarding the antifungal activity of the Cu-particles, data in Fig. 1b showed a remarkable growth inhibition of A. flavus, A. niger, P. chrysogenum, F. solani and A. solani. The fungal growth was completely disappeared at the concentration 250 μg/mL while at 200 μg/mL the recorded percentage of the inhibition was 95.7 % for A. niger, 95.2 % for F. solani and 97.4 % for A. solani. At the same time, data in Table 1 and Fig. 2 demonstrated the antifungal activity of Cu-particles in comparison to CuSO4. Generally, the antifungal activities of copper were enhanced by increasing their concentration and Cu-particles recorded higher activities than those of CuSO4. The maximum growth reduction was recorded at 200 μg/mL of Cu-particles against A. flavus (67.7 %) and F. solani (64.3 %) while the lowest growth inhibition was recognized with A. niger (60.7 %).

Antimicrobial activity of the stones treated with Cu/polymer composites

Although elevated concentrations of Cu-particles demonstrated superior antimicrobial potentialities, silicon and acrylic polymers were functionalized with 150 μg/mL of Cu-particles in order to minimize color change of the treated stones. Data in Table 2 showed the antibacterial activities of the treated stones against E. coli, S. parvulus and B. subtilis. The treated sandstone blocks recorded a clear reduction in the percentage of the bacterial cell recovery; 90.1 & 89.1 % for E. coli, 95.2 & 92.0 % for S. parvulus and 95.3 & 94.8 % for B. subtilis with the functionalized silicon and acrylic polymers, respectively. At the same time, the treated limestone blocks demonstrated a clear suppression in the percentage of cell recovery of E. coli (93.1 & 86.8 %), S. parvulus (93.4 & 95.2 %) and B. subtilis (93.1 & 95.0 %) for the functionalized silicon and acrylic polymers, respectively.

Table 2 Bacterial cell recovery from sandstone and limestone blocks treated with silicon (S) and acrylic (AC) polymers impregnated with copper nanoparticles
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Physical and mechanical properties of the treated stones

Results in Table 3 demonstrated a clear improvement in the physical and mechanical properties of the tested stones as a result of the application of the consolidation polymers. Silicon and acrylic polymers showed an increase in the percentage of bulk density, compressive strength and tensile strength of the treated stones. Meanwhile the water absorption capacity and porosity of the treated stones were sharply reduced as a result of using the consolidation polymers.

Table 3 Physical and mechanical properties of sandstone and limestone samples treated with the functionalized silicone (S) and acrylic (AC) polymers
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SEM & EDX analysis of the composite Cu-particles based on polymers

The current study Fig. 3 showed the analysis of the treated and untreated polymers with composite copper structures by scanning electron microscope. In case of the functionalized polymers, tiny particles (10–50 nm in diameter) were identified while these particles were absent in the un-functionalized polymers. At the same time, the EDX analysis of these minute structures showed the presence of the elemental copper in the treated silicon and acrylic polymers in addition to oxygen, carbon, sulfur, silicon, potassium, chloride and calcium elements. Furthermore, the EDX analysis of the un-functionalized polymers showed the presence of carbon, silicon and oxygen elements with the silicon polymer while the peaks of carbon, oxygen, silicon, chloride, calcium and aluminum peaks were recognized in case of the acrylic polymer.

Fig. 3
figure 3

SEM images and EDX analysis of the unfunctionalized silicon polymer (a), silicon polymer functionalized with CuNPs (b) and unfunctionalized acrylic polymer (c), acrylic polymer functionalized with CuNPs (d) while black arrows indicate the elemental copper

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Discussion

In our previous study [27], the capability of some bacterial strains for the precipitation of various metal ions out of their solutions was recorded using the culture biogas. In the present work, Cu-particles were prepared biologically via exposing the copper ions to the biogenic volatiles released during the aerobic growth of Escherichia coli Z1. One of the main constitutes of these gases is ammonia that is responsible for the transformation of copper ions into nitrogen-based copper particles [30]. The existence of ammonia in bacterial biogas is mainly attributed to the catabolic reactions of some organic matter [31].

At minor concentration of ammonia, the aqueous copper sulfate solution produces copper hydroxide while high ammonia levels induces the formation of diammine copper (II) complex [Cu(NH3)2]+ [27]. In fact, the alteration of the copper ions into colloidal copper particles is correlated with the exposure time. At short exposure time, minute copper particles (10–50 nm) were formed as showed in the SEM analysis. The EDX analysis of the copper structures clarified the existence of sulfur that could be attributed to the incidence of volatile organothiol compounds in the bacterial biogas [32].

This study clarified a marked antimicrobial efficacy of CuNPs against various bacterial and fungal species. The biocidal activity of CuNPs could be attributed to the effect of the CuNPs and/or the copper ions discharged from CuNPs. Because of the great surface area of the nanoparticles, it could be tightly adsorbed onto the surface of the microbial cells resulting in; i) disruption of cell permeability and release of integral components [33], ii) denaturing of some functional biomolecules [10, 13], iii) induction of oxidative damage to the microbial cells. However, some studies have reported that the liberated Cu2+ is the motivating force behind the antimicrobial properties of polymers containing Cu-nanocomposites [2, 34]. At the same time, the discharged copper ions might be moved inside the microbial cells or attached to their outer surfaces resulting in cell apoptosis via protein denaturation and disruption of cell membrane [35, 36]. Obviously, nonspecific mode of action of Cu2+ or CuNPs against bacteria and fungi makes them perfect antimicrobial agents with low possibility of developing microbial resistance [4, 33].

In the current study, the silicon and acrylic polymers that were loaded with the copper nanoparticles showed a positive influence on the treated stones through suppressing the growth of tested bacterial strains at various levels. At the same time, the antimicrobial activity of the biosynthesized CuNPs was not changed by merging with consolidation polymers. These results are in agreement with our preceding study [19] that revealed the protection of some archeological stone against microbial colonization via the application of consolidation polymers/AgNPs composites onto their surfaces. Similarly, Pinna et al. [37] clarified a superior protective behavior against the microbial colonization on stones via treating them with consolidants loaded with copper nanoparticles.

In addition to the antimicrobial task of the functionalized polymers, they demonstrated an apparent perfection in the physical and mechanical properties of the treated stones. The consolidation polymers especially silicone polymer decreased the level of water absorption and porosity of stones through the formation of a protective layer. This layer is formed due to the penetration of the polymer molecules into voids and pores of the stone matrix. Moreover, the mechanical measurements indicated that both types of polymers increased the compressive strength which reflects the importance of using these polymers in the consolidation processes of the limestone monuments. These results are in harmony with Ahmed [38] who recorded a marked improvement in the physico-mechanical behavior of limestone samples after treating them with some synthetic polymers. Also, Khallaf et al. [39] showed an increase in bulk density and decrease in porosity as well as increase in compressive strength of the monuments made of sandstone and limestone after treating them with some organic polymers.

Conclusion

Copper has strong biocidal activity with non-specific mode of action against microbial cells that make it ideal antimicrobial agent. CuNPs were prepared through novel bioprocess that utilizes volatile metabolites of Escherichia coli to aggregate Cu ions into nanometal structures away from the bacterial cells. This bioprocess is inexpensive and eco-friendly. Besides, uncontaminated bacterial biomass could be used safely in different applications. The incorporation of CuNPs into polymer matrix produced nanocopper composites with remarkable antimicrobial capability. The functionalized consolidation polymers could be used not only to inhibit the microbial growth on the surfaces of historical stones but also to improve physical and mechanical properties of the treated stones. Additional research is required to evaluate the application of consolidation polymers loaded with nanoparticles of copper in situ treatment.

References

  1. Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Rivera B, et al. Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci U S A. 2003;100:13549–54.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Delgado K, Quijada R, Palma R, Palza H. Polypropylene with embedded copper metal or copper oxide nanoparticles as a novel plastic antimicrobial agent. Lett Appl Microbiol. 2011;53:50–4.

    Article  CAS  PubMed  Google Scholar 

  3. Duran N, Marcato PD, De Souza GI, Alves OL, Esposito E. Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. J Biomed Nanotechnol. 2007;3:203–8.

    Article  CAS  Google Scholar 

  4. Anyaogu KC, Fedorov AV, Neckers DC. Synthesis, characterization, and ntifouling potential of functionalized copper nanoparticles. Langmuir. 2008;24:4340–6.

    Article  CAS  PubMed  Google Scholar 

  5. Chatterjee AK, Sarkar RK, Chattopadhyay AP, Aich P, Chakraborty R, Basu T. A simple robust method for synthesis of metallic copper nanoparticles of high antibacterial potency against E. coli. Nanotechnol. 2012;23:1–11.

    Article  Google Scholar 

  6. Jung, JH, Kim SW, Min JS, Kim YJ, Lamsal K, Kim KS, Lee YS. The effect of nanosilver liquid against the white rot of green onion caused by Sclerotium cepivorum. Mycobiol. 2010;38:39–45.

  7. Lamsal K, Kim SW, Jung JH, Kim YS, Kim KS, Lee YS. Inhibition effects of silver nanoparticles against powdery mildews on cucumber and pumpkin. Mycobiology. 2011;39(1):26–32.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Kim SW, Jung JH, Lamsal K, Min JS, Lee YS. Antifungal Effects of Silver Nanoparticles (AgNPs) against Various Plant Pathogenic Fungi. Mycobiol. 2012;40(1):53–8.

    Article  CAS  Google Scholar 

  9. Ouda SM. Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Res J Microbiol. 2014;9:34–42.

    Article  CAS  Google Scholar 

  10. Ruparelia JP, Chatterjee A, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater. 2008;4:707–16.

    Article  CAS  PubMed  Google Scholar 

  11. Theivasanthi T, Alagar M. Studies of copper nanoparticles effects on microorganisms. Ann Bio Res. 2011;2:368–73.

    CAS  Google Scholar 

  12. Ramyadevi J, Jeyasubramanian K, Marikani A, Rajakumar G, Rahuman AA. Synthesis and antimicrobial activity of copper nanoparticles. Materials Lett. 2012;71:114–6.

    Article  CAS  Google Scholar 

  13. Wei Y, Chen S, Kowalczyk B, Huda S, Gray TP, Grzybowski BA. Synthesis of stable, low dispersity copper nanoparticles and nanorods and their antifungal and catalytic properties. J Phys Chem. 2010;114(37):15612–6.

    CAS  Google Scholar 

  14. Usman MS, ElZowalaty ME, Shameli K, Zainuddin N, Salama M, Ibrahim NA. Synthesis, characterization, and antimicrobial properties of copper nanoparticles. Int J Nanomedicine. 2013;8:4467–79.

    PubMed  PubMed Central  Google Scholar 

  15. Simkiss K, Wilbur KM. Biomineralization. New York: Academic; 1989.

    Google Scholar 

  16. Mann S. Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry. Oxford: Oxford University Press; 2001.

    Google Scholar 

  17. Zhang X, Yan SR, Tyagi RD, Surampalli RY. Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere. 2011;82(4):489–94.

    Article  CAS  PubMed  Google Scholar 

  18. Capeness MJ, Edmundson MC, Horsfall LE. Nickel and platinum group metal nanoparticle production by Desulfovibrio alaskensis G20. New Biotechnol. 2015;6:727–31.

    Article  Google Scholar 

  19. Essa AMM, Khallaf MK. Biological nanosilver particles for the protection of archaeological stones against microbial colonization. Int Biodeterior Biodegrad. 2014;94:31–7.

    Article  CAS  Google Scholar 

  20. Bhattacharya R, Mukherjee P. Biological properties of “naked” metal nanoparticles. Adv Drug Deliv Rev. 2008;60(11):1289–306.

    Article  CAS  PubMed  Google Scholar 

  21. Dakal TC, Cameotra SS. Geomicrobiology of cultural monuments and artworks: mechanism of biodeterioration, bioconservation strategies and applied molecular approaches. In: Mason AC, editor. Bioremediation: Biotechnology, Engineering, and Environment Management. New York: Nova Science Publishers; 2011.

    Google Scholar 

  22. Pippo F, Bohn A, Congestri R, De Philippis R, Albertano P. Capsular polysaccharides of cultured phototrophic biofilms. Biofouling. 2009;25:495–504.

    Article  PubMed  Google Scholar 

  23. Warscheid T, Braams J. Biodeterioration of stone: a review. Int Biodeterior Biodegrad. 2000;46:343–68.

    Article  CAS  Google Scholar 

  24. La Russa MF, Ruffolo SA, Rovella N, et al. Multifunctional TiO2 coatings for cultural heritage. Prog Org Coatings. 2012;74:186–91.

    Article  Google Scholar 

  25. Ruffolo SA, Macchia A, La Russa MF, Mazza L, Urzì C, De Leo F, et al. Marine antifouling for underwater archaeological sites: TiO2 and Ag-Doped TiO2. Int J Photoenergy. 251647, 6. http://0-dx-doi-org.brum.beds.ac.uk/10.1155/2013/251647.

  26. Essa AM. The effect of continuous mercury stress on mercury reducing community of some characterized bacterial strains. Afr J Microbiol Res. 2013;6(6):1255–61.

    Google Scholar 

  27. Essa AM, Abd-Alsalam ES, Ali RM. Biogenic volatile compounds of activated sludge and their application for metal bioremediation. Afr J Biotechnol. 2012;11(42):9993–10001.

    CAS  Google Scholar 

  28. Perez C, Pauli M, Bazevque P. An antibiotic assay by the agar well diffusion method. Acta Biologiae Medicine Experimentalis. 1990;5:113–5.

    Google Scholar 

  29. Fiori ACG, Schwan-Estrada KRF, Stangarlin KRF, Vida JB, Scapim CA, Cruz MES, Pascholati S. Antifungal activity of leaf extract and essential oils of some medicinal plants against Didymella bryoniae. J Phytopathol. 2000;148:483–7.

  30. Macaskie LE, Creamer NJ, Essa AM, Brown NL. A new approach for the recovery of precious metals from solution and from leachates derived from electronic scrap. Biotechnol Bioeng. 2007;96:631–9.

    Article  CAS  PubMed  Google Scholar 

  31. Kuok F, Mimoto H, Nakasaki K. Reduction of ammonia inhibition of organic matter degradation by turning during a laboratory-scale swine manure composting. Int J Waste Resour. 2013;3:518–27.

    Article  Google Scholar 

  32. Essa AM, Macaskie LE, Brown NL. A new method for mercury removal. Biotechnol Lett. 2005;27(21):1649–55.

    Article  CAS  PubMed  Google Scholar 

  33. Raffi M, Mehrwan S, Bhatti TM, Akhter JI, Hameed A, Yawar W, et al. Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Ann Microbiol. 2010;60(1):75–80.

    Article  CAS  Google Scholar 

  34. Palza, H, Gutierrez S, Delgado K, Salazar O, Fuenzalida V, Avila J, Figueroa G, Quijada R. Toward tailor-made biocide materials based on polypropylene/copper nanoparticles. Macromol Rapid Commun. 2010;31:563–9.

  35. Palza H. Antimicrobial polymers with metal nanoparticles. Int J Mol Sci. 2015;19(1):2099–116.

    Article  Google Scholar 

  36. Lin YE, Vidic RD, Stout JE, Mccartney CA, Yu VL. Inactivation of Mycobacterium avium by copper and silver ions. Water Res. 1998;32(7):1997–2000.

    Article  CAS  Google Scholar 

  37. Pinna D, Salvadori B, Galeotti M. Monitoring the performance of innovative and traditional biocides mixed with consolidants and water-repellents for the prevention of biological growth on stone. Sci Total Environ. 2012;423:132–41.

    Article  CAS  PubMed  Google Scholar 

  38. Ahmed HT. Physical and mechanical characteristics of Helwan limestone: For conservation treatment of ancient Egyptian limestone monuments. J Am Sci. 2015;11(2):136–49.

    Google Scholar 

  39. Khallaf MK, El-Midany AA, El-Mofly SE. Influence of acrylic coatings on the interfacial, physical, and mechanical properties of stone-based monuments. Prog Org Coat. 2011;72:592–8.

    Article  CAS  Google Scholar 

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Acknowledgement

The authors wish to thank Dr. Refat Mohamed Ali, Prof. of Plant Physiology, Botany Department, Faculty of Science, Fayoum University, for his great support and valuable suggestions. We also gratefully acknowledge all the staff of Botany Department, Faculty of Science, Fayoum University.

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All relevant data are within the paper.

Authors' contributions

AMME conceived and designed the study, performed preparation of Cu-particles, antimicrobial tests, analyzed the data obtained and drafted this paper. MKK provided stone samples and consolidant polymers in addition to physical and chemical analysis of stones. AMME and MKK performed the SEM and EDX analysis. All authors read and approved the final manuscript.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Fayoum University, Fayoum, Egypt

    Ashraf M. M. Essa

  2. Biology Department, Faculty of Science, Jazan University, Jazan, Saudi Arabia

    Ashraf M. M. Essa

  3. Conservation Department, Faculty of Archaeology, Fayoum University, Fayoum, Egypt

    Mohamed K. Khallaf

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Essa, A.M.M., Khallaf, M.K. Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles. BMC Microbiol 16, 144 (2016). https://0-doi-org.brum.beds.ac.uk/10.1186/s12866-016-0766-8

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