Fig. 4
a. Liposomes simulating the B. burgdorferi B31 OM were incubated with recombinant BB0405, BB0406, E. coli OmpA, or GST and then separated on discontinuous sucrose gradients. Gradient fractions were collected from the top (TF) and bottom (BF) and subjected to immunoblot with appropriate antibodies. b. Liposomes loaded with the fluorophore Tb(DPA)3 3− were incubated with recombinant B. burgdorferi B31 BB0405, BB0406, P66, E. coli OmpF, or E. coli OmpA in buffer supplemented with EDTA, and fluorophore efflux was measured as quenched fluorescence. Three independent experiments were compared and each bar represents the mean ± S.E. (error bars). Statistical significance compared with E. coli OmpA is indicated with * (p < 0.05)