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Fig. 3 | BMC Microbiology

Fig. 3

From: Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes

Fig. 3

AAT, C and ΔAAT did not directly target viral RNA reverse transcription. a CD4+ T cells were pretreated with or without AAT/C/ΔAAT and then infected by HIV-1NL4.3 without removing the reagents. Infected CD4+ T cells were then washed to remove unbound viruses and reagents and then incubated for 1, 6, 12, 18, 24, or 30 h in the presence or absence of AAT/C/ΔAAT (the same condition as before infection) to isolate whole cell proteins with viral proteins. The activity of HIV-1 reverse transcriptase in normalized whole extracts was detected following the protocol described in the Materials and Method. b CD4+ T cells were also infected with HIV-1NL4.3 without pretreatment and then incubated with the presence or absence of AAT, C, ΔAAT or HIV-1 reverse transcriptase inhibitor emtricitabine (10−3 g/L). After 0, 5, 11, 17, 23, or 29 h’ incubation, the whole cell proteins with viral proteins were extracted from these CD4+ T cells and normalized to detect the activity of HIV-1 reverse transcriptase. Ct: control group without reagent treatment; FTC: emtricitabine treatment

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