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Fig. 4 | BMC Microbiology

Fig. 4

From: Auxotrophic Actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (NAD) supplementation

Fig. 4

Confirmation of the presence of A. pleuropneumoniae in multi-species biofilms. Confirmation of A. pleuropneumoniae in the dual-species biofilm of A. pleuropneumoniae 719 and S. suis 735 or B. bronchiseptica 276 by FISH with an ApxIVAN-AlexaFluor 633 probe (red). Images of the X-Z plane of biofilm of single and dual-species biofilms grown in BHI with or without NAD. Bacterial cell in the biofilms were stained with FilmTracer ™ FM ® 1-43 (Molecular Probes) which are represented in green. Yellow represent the co-localization of both the ApxIVAN probe and the stain FM 1-43. App: A. pleuropneumoniae; Ss: S. suis; Bb: B. bronchiseptica

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BMC Microbiology

ISSN: 1471-2180

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