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Fig. 3 | BMC Microbiology

Fig. 3

From: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity

Fig. 3

Phenotype detection of the P. multocida Δcrp mutant. a Analysis of the growth curve. The parent strain, S416 (Δcrp) and S416 (pQK176) were grown in BHI broth or BHI broth supplemented with kanamycin, and the OD600 values were measured every 2 h over a period of 14 h. The data are expressed as the means ± SD, and the asterisks indicate significant differences compared with the parent strain. b and c Profiles of the OMPs and LPS of P. multocida. OMPs or LPSs were isolated from the parent strain or the Δcrp mutant and then analyzed by SDS-PAGE. Coomassie blue staining and silver staining were then performed to visualize the OMPs (b) and LPSs (c), respectively. M refers to the protein marker

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BMC Microbiology

ISSN: 1471-2180

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