| Ion Linker | Barcode | Spacer | Primer | Ref |
---|---|---|---|---|---|
V4-V5 | Â | Â | Â | Â | [23] |
 Forward barcoded primers |  |  |  |  |  |
  Mock DNA | CCATCTCATCCCTGCGTGTCTCCGACTCAG | TCCCTTGTCTCC | GT | GTGCCAGCMGCCGCGGTAA |  |
  PBS Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ACGAGACTGATT | GT | GTGCCAGCMGCCGCGGTAA |  |
  PBS RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | GCTGTACGGATT | GT | GTGCCAGCMGCCGCGGTAA |  |
  Glycerol Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATCACCAGGTGT | GT | GTGCCAGCMGCCGCGGTAA |  |
  Glycerol RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | TGGTCAACGATA | GT | GTGCCAGCMGCCGCGGTAA |  |
 Reverse primer | CCTCTCTATGGGCAGTCGGTGAT |  | CC | CCGTCAATTYYTTTRAGTTT |  |
V1-V2 set 1 | Â | Â | Â | Â | [4] |
 Forward barcoded primers |  |  |  |  |  |
  Mock DNA | CCATCTCATCCCTGCGTGTCTCCGACTCAG | TGCATACACTGG | GT | AGAGTTTGATCCTGGCTCAG |  |
  PBS Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGTCGAACGAGG | GT | AGAGTTTGATCCTGGCTCAG |  |
  PBS RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ACCAGTGACTCA | GT | AGAGTTTGATCCTGGCTCAG |  |
  Glycerol Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | GAATACCAAGTC | GT | AGAGTTTGATCCTGGCTCAG |  |
  Glycerol RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | GTAGATCGTGTA | GT | AGAGTTTGATCCTGGCTCAG |  |
 Reverse primer | CCTCTCTATGGGCAGTCGGTGAT |  | CC | TGCTGCCTCCCGTAGGAGT |  |
V1-V2 set 2 | Â | Â | Â | Â | [17] |
 Forward barcoded primers |  |  |  |  |  |
  Mock DNA | CCATCTCATCCCTGCGTGTCTCCGACTCAG | GCGATATATCGC | GT | AGMGTTYGATYMTGGCTCAG |  |
  PBS Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CGAGCAATCCTA | GT | AGMGTTYGATYMTGGCTCAG |  |
  PBS RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGTCGTGCACAT | GT | AGMGTTYGATYMTGGCTCAG |  |
  Glycerol Qiagen | CCATCTCATCCCTGCGTGTCTCCGACTCAG | GTATCTGCGCGT | GT | AGMGTTYGATYMTGGCTCAG |  |
  Glycerol RBB | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CGAGGGAAAGTC | GT | AGMGTTYGATYMTGGCTCAG |  |
 Reverse primer | CCTCTCTATGGGCAGTCGGTGAT |  | CC | TGCTGCCTCCCGTAGGAGT |  |