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Table 2 Key characteristics of the SBSEC-MLST scheme for S. equinus, Sgg and Sgm

From: Phylogenetic, epidemiological and functional analyses of the Streptococcus bovis/Streptococcus equinus complex through an overarching MLST scheme

 

S. equinus (n = 10)

Sgg (n = 6)

Sgm (n = 7)

Index of association1

IA

2.092

2.093

0.329

IA S

0.232

0.233

0.037

Gene

Gene product

A3 [bp]

A [no]

dN/dS

pms [No (%)]

SID

A [no]

dN/dS

pms [No (%)]

SID

A [no]

dN/dS

pms [No (%)]

SID

ddlA

D-alanine-D-alanine ligase

489

8

0.093

52 (10.6)

0.956 (0.894–1.018)

6

0.098

8 (1.6)

1.000 (NaN7-NaN)

2

2.070

4 (0.8)

0.286 (-0.092–0.664)

gki

glucokinase

438

8

0.061

32 (7.3)

0.956 (0.894–1.018)

3

0.000

6 (1.4)

0.600 (0.215–0.985)

3

−6

6 (1.4)

0.714 (0.563–0.865)

glnA

glutamine synthetase

396

2

0.000

1 (0.3)

0.200 (-0.104–0.504)

2

0.000

3 (0.8)

0.600 (0.600–0.600)

1

−6

0 (0)

0 (0.000–0.000)

mutS

DNA mismatch repair ATPase

552

10

0.015

43 (7.8)

1.000 (NaN7-NaN)

3

0.037

17 (3.1)

0.600 (0.215–0.985)

4

0.123

6 (1.1)

0.810 (0.630–0.989)

mutS2

mismatch repair ATPase

495

10

0.010

28 (5.7)

1.000 (NaN7-NaN)

5

0.017

13 (2.6)

0.933 (0.805–1.062)

2

0.000

2 (0.4)

0.571 (0.465–0.678)

pheS

phenylalanyl tRNA synthetase

480

8

0.075

14 (2.9)

0.956 (0.894–1.018)

3

0.000

11 (2.3)

0.600 (0.215–0.985)

2

−6

1 (0.2)

0.286 (-0.092–0.664)

proS

prolyl tRNA synthetase

417

9

0.046

59 (14.1)

0.978 (0.927–1.028)

4

0.000

5 (1.2)

0.800 (0.528–1.072)

1

−6

0 (0)

0 .000 (0.000–0.000)

pyrE

orotate phosphoribosyl transferase

393

7

0.040

21 (5.3)

0.933 (0.871–0.995)

5

0.000

4 (1)

0.933 (0.805–1.062)

3

0.042

16 (4.1)

0.762 (0.655–0.869)

thrS

threonyl tRNA synthetase

573

7

0.000

17 (3)

0.933 (0.871–0.995)

4

0.032

18 (3.1)

0.800 (0.528–1.072)

2

0.096

87 (15.2)

0.286 (-0.092–0.664)

tpiA

triosephosphate isomerase

408

3

0.049

7 (1.7)

0.511 (0.200–0.822)

2

0.000

1 (0.2)

0.533 (0.277–0.790)

2

0.000

1 (0.2)

0.476 (0.183–0.769)

Mean (95 % CI)

    

0.842 (0.307–1.378)

   

0.74 (0.393–1.087)

   

0.419 (−0.173–1.011)

  1. 1Index of association based on one strain per ST: IA: calculated using classical Maynard Smith approach, IA S: standardized according to Haubold
  2. 2SBSEC: includes also 3 S. gallolyticus subsp. pasteurianus and 1 S. alactolyticus strains not listed separately
  3. 3 A allele
  4. 4 Pms polymorphic sites
  5. 5SID (95 % CI): Simpson’s index of diversity with 95 % confidence interval (CI) calculated from SID+/− 2*Standard deviation
  6. 6no dN/dS calculation due to limited allele numbers and differences
  7. 7NaN: maximum diversity, no CI calculcated. Values were calculated for the entire SBSEC and individual (sub)species, including all 10 target genes and Simpson’s index of diversity (SID) as an indication for the differentiation power among strains