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Table 1 Key characteristics of the SBSEC-MLST scheme for the overall SBSEC, Sii and S. lutetiensis

From: Phylogenetic, epidemiological and functional analyses of the Streptococcus bovis/Streptococcus equinus complex through an overarching MLST scheme

  SBSEC (n = 74)2 Sii (n = 39) S. lutetiensis (n = 8)
Index of association1 IA 3.132 2.079 2.422
IA S 0.349 0.231 0.269
Gene Gene product A3 [bp] A3 [no] dN/dS pms4 [No (%)] SID (95 % CI)5 A [no] dN/dS pms [No (%)] SID A [no] dN/dS pms [No (%)] SID
ddlA D-alanine-D-alanine ligase 489 28 0.079 143 (29.2) 0.905 (0.867–0.943) 5 0.171 5 (1) 0.704 (0.648–0.761) 6 0.123 18 (3.7) 0.929 (0.840–1.017)
gki glucokinase 438 27 0.059 119 (27.2) 0.901 (0.858–0.944) 5 0.144 5 (1.1) 0.668 (0.584–0.752) 5 0.072 21 (4.8) 0.893 (0.722–1.064)
glnA glutamine synthetase 396 21 0.039 59 (14.9) 0.906 (0.878–0.934) 7 0.163 8 (2) 0.745 (0.688–0.802) 6 0.064 10 (2.5) 0.929 (0.806–1.052)
mutS DNA mismatch repair ATPase 552 30 0.028 172 (31.2) 0.926 (0.894–0.959) 5 0.066 4 (0.7) 0.752 (0.688–0.815) 5 0.055 39 (7.1) 0.857 (0.704–1.010)
mutS2 mismatch repair ATPase 495 36 0.030 159 (32.1) 0.93 (0.890–0.969) 10 0.263 10 (2) 0.762 (0.656–0.869) 5 0.022 44 (8.9) 0.857 (0.704–1.010)
pheS phenylalanyl tRNA synthetase 480 28 0.026 110 (22.9) 0.923 (0.889–0.957) 10 0.009 51 (10.6) 0.776 (0.680–0.872) 4 0.015 18 (3.8) 0.643 (0.301–0.985)
proS prolyl tRNA synthetase 417 28 0.059 128 (30.7) 0.92 (0.887–0.953) 7 0.353 7 (1.7) 0.756 (0.678–0.833) 5 0.106 18 (4.3) 0.786 (0.521–1.051)
pyrE orotate phosphoribosyl transferase 393 36 0.022 97 (24.7) 0.972 (0.961–0.983) 16 0.061 22 (5.6) 0.927 (0.894–0.960) 5 0.013 22 (5.6) 0.857 (0.704–1.010)
thrS threonyl tRNA synthetase 573 31 0.074 168 (29.3) 0.957 (0.941–0.974) 11 0.105 99 (17.3) 0.883 (0.842–0.924) 5 0.061 27 (4.7) 0.857 (0.704–1.010)
tpiA triosephosphate isomerase 408 17 0.119 36 (8.8) 0.894 (0.862–0.927) 4 0.152 3 (0.7) 0.687 (0.637–0.737) 5 0.056 12 (2.9) 0.893 (0.816–0.969)
Mean (95 % CI)      0.924 (0.874–0.973)     0.766 (0.602–0.93)     0.85 (0.682–1.018)
  1. 1Index of association based on one strain per ST: IA: calculated using classical Maynard Smith approach, IA S: standardized according to Haubold
  2. 2SBSEC: includes also 3 S. gallolyticus subsp. pasteurianus and 1 S. alactolyticus strains not listed separately
  3. 3 A allele
  4. 4 Pms polymorphic sites
  5. 5SID (95 % CI): Simpson’s index of diversity with 95 % confidence interval (CI) calculated from SID+/− 2*Standard deviation
  6. 6no dN/dS calculation due to limited allele numbers and differences
  7. Values were calculated for the entire SBSEC and individual (sub)species, including all 10 target genes and Simpson’s index of diversity (SID) as an indication for the differentiation power among strains