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Fig. 5 | BMC Microbiology

Fig. 5

From: The calcineruin inhibitor cyclosporine a synergistically enhances the susceptibility of Candida albicans biofilms to fluconazole by multiple mechanisms

Fig. 5

Calcineurin inhibitor CsA and FLC synergistically increase intracellular calcium concentration clinical C. albicans isolates. Clinical C. albicans isolates were exposed to 32 μg/mL FLC or 75 μg/mL of CsA alone, or a combination of CsA and FLC for 24 h prior to be used for determining intracellular calcium concentration by a flow cytometric assay (FACS). a Representative plot images of FACS analysis showed the fraction of cells with high intracellular calcium concentration (M2 fraction) treated with indicated conditions. b Quantitative analysis of percentages of cells with high intracellular calcium levels. The result showed that both CsA and FLC alone could reduce intracellular calcium, but a combination of them caused a time-dependently evoked intracellular calcium concentration. Compared with the untreated group of each indicated time point, *: p < 0.05; **: p < 0.01. Data represented the mean ± SD from three independent triplicated experiments (N = 9)

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BMC Microbiology

ISSN: 1471-2180

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