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Table 5 Amount of metabolized 22:6n-3 (μg/tube) recovered in FAME peaks. Incubation was performed in growth medium containing autoclaved-centrifuged rumen fluid (20 % v/v) in the presence of 22:6n-3 (20 μg/mL) with Butyrivibrio proteoclasticus P18. Tubes were withdrawn at 0, 2, 4, 8, 12 and 24 h incubation to study the extent of 22:6n-3 biohydrogenation. Peak numbers correspond to peaks in Fig. 2

From: Biohydrogenation of 22:6n-3 by Butyrivibrio proteoclasticus P18

Peaks

 

Incubation duration (h)

4

8

12

24

1

Δ7, Δ10, Δ13, Δ16, Δ19-22:5

5.51 ± 0.59

19.55 ± 2.82

13.72 ± 3.93

-

2

Δ4, Δ10, Δ13, Δ16, Δ19-22:5

-

3.68 ± 1.22

5.21 ± 3.88

-

3

Δ5, Δ10, Δ13, Δ16, Δ19-22:5

4.41 ± 1.10

53.32 ± 8.58

28.37 ± 39.9

-

4

Δ10, Δ13, Δ16, Δ19-22:4

 

11.34 ± 3.05

91.90 ± 40.6

26.41 ± 36.1

5

Δ13, Δ16, Δ20-22:3

 

6.89 ± 1.25

11.86 ± 3.27

9.34 ± 5.26

6

Δ13, Δ16, Δ19-22:3

 

-

10.26 ± 5.80

38.69 ± 3.97

7

Δ13, Δ16, Δ20-22:3

 

-

0.36 ± 0.32

6.75 ± 5.59

8

Δ11, Δ16, Δ19-22:3

 

-

10.10 ± 6.52

15.73 ± 16.6

9

Δ16, Δ20-22:2

 

-

0.34 ± 0.30

7.36 ± 7.36

10

Δ16, Δ19-22:2

 

-

1.38 ± 1.21

26.68 ± 11.4

11

Δ16, Δ20-22:2

 

-

-

10.09 ± 10.8

12

Δ14, Δ18-22:2

 

-

-

6.69 ± 5.96