Table 1 Overview of the in vitro experiments conducted in this study
From: Biohydrogenation of 22:6n-3 by Butyrivibrio proteoclasticus P18
Exp. | Autoclaved-rumen fluid/tube (%) | Bacteriaa | 22:6n-3 (μg/mL) | Gas phaseb | Incubation period (h) |
|---|---|---|---|---|---|
Uncentrifuged | |||||
1 | 20 and 50 | B. fibri | 20 | CO2 | 0 and 48 |
2 | 50 | B. proteo | 20 | CO2, H2 and N2 | 0 and 48 |
3 | 50 | B. proteo | 20 | H2 | 0, 2, 4, 8, 12, 24 and 48 |
4 | 50 | B. proteo | 5, 10, 40 and 80 | H2 | 0 and 48 |
5c | 50 | B. proteo | 10 and 40 | H2 | 0, 2, 4, 8, 12, 24 and 48 |
Centrifuged | |||||
6 | 20 and 50 | B. fibri | 0.5, 1, 2, 4, 8, 16 and 32 | CO2 | 0 and 48 |
7 | 20 | B. proteo | 20 | H2 | 0 and 24 |
8 | 20 | B. proteo | 20 | H2 | 0, 2, 4, 8, 12 and 24 |