Table 5 Primers for PCR amplification and DNA sequencing
Gene | Amplified Region (bp) | Orientation | Oligonucleotide sequence (5′-->3′) | Tm (°C) |
|---|---|---|---|---|
katG | 580 to 1257 | Forward | GATGAGGTCTATTGGGGCAAG | 59.8 |
Reverse | GTCTCGGTGGATCAGCTTGTA | 59.8 | ||
mabA-inhA promoter | −436 to 182 (mabA) | Forward | ATGCGCTCTTCCCAGACTT | 58.0 |
Reverse | TCACATTCGACGCCAAACAG | 60.0 | ||
OxyR ’ -ahpC intergenic region | 285 (oxyR ’) to 312 (ahpC) | Forward | CCCTCATGCAGTCACAACAA | 60.0 |
Reverse | TTTGAGGTCGTTGTGCTGTG | 60.0 | ||
rpoB RRDR | 916 to 1572 | Forward | GGTCGCTATAAGGTCAACAAGAAG | 61.0 |
Reverse | GTACACGATCTCGTCGCTAACC | 62.1 | ||
embB | 847 to 1581 | Forward | GTGATATTCGGCTTCCTGCTCT | 60.2 |
Reverse | GTAGTAGTAACGCAGGTTCTCGGTA | 62.9 | ||
rpsL | 254 to 877 | Forward | GAATCGAGTTTGAGGCAAGCTAT | 58.8 |
Reverse | CTCAAGCGCACCATAAACAAT | 55.9 |