Prevalence of plasmid-bearing and plasmid-free Chlamydia trachomatis infection among women who visited obstetrics and gynecology clinics in Malaysia

Table 4 PCR primers used in C. trachomatis conventional PCR and real-time PCR diagnosis. For nested PCR amplification of Momp and plasmid, outer primer pairs were used at first round PCR, while inner primer pairs were used at second round PCR amplification. For real-time PCR diagnosis, Momp inner primer pairs, plasmid Pgp8 inner primer pairs and plasmid Pgp1 primer pairs were used

Target genes	Primer	Sequence (5′-3′)	Amplicon size
Momp	Outer forward	TTGTTTTCGACCGTGTTTTG	455 bp
	Outer reverse	AGCRTATTGGAAAGAAGCBCCTAA
	Inner forward	AAACWGATGTGAATAAAGARTT	395 bp
	Inner reverse	TCCCASARAGCTGCDCGAGC
Plasmid Pgp8	Outer forward	TTGGCYGCTAGAAAAGGCGATT	212 bp
	Outer reverse	TCCGGAACAYATGATGCGAAGT
	Inner forward	AACCAAGGTCGATGTGATAG	150 bp
	Inner reverse	TCAGATAATTGGCGATTCTT
Plasmid Pgp1	Forward	TTCTTTGATGGCTTCCCAAC	456 bp
	Reverse	ACGATTTTCTCCAACCGATG
β-globin	Forward	GAAGAGCCAAGGACAGGTAC	268 bp
	Reverse	CAACTTCATCCACGTTCACC

ISSN: 1471-2180