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Table 3 Sequences of primers used in this study

From: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme

Primer

Sequence

Description

5′-label

abs (nm)

em [nm]

fV

5′- GTAGGTGGCGCAGTTCGAG -3′

5′- GTGGGTGGAGCGGTAAGAG -3′

Degenerated forward primer for motif A region of V. alginolyticus/V. parahaemolyticus (sequence 1) and V. vulnificus (sequence 2)

   

rValg

5′- ACCAGTGTAACCAGAACCTGAC -3′

Reverse primer for loop sequence of V. alginolyticus

ATTO 425

436

484

rVpar

5′- TCCTCTTCTAGCGTTACGCTTG -3′

Reverse primer for loop sequence of V. parahaemolyticus

ATTO 532

532

553

rVvul

5′- CACATCTGGGGAGAAAAAGCAC -3′

Reverse primer for loop sequence of V. vulnificus

ATTO 633

629

657

fA

5′- GGGTGAGGGACAAGCTG -3′

5′- GGGTGCGGGACAAGCTG -3′

Degenerated forward primer for motif A region of A. fumigatus/A. niger (sequence 1) and A. terreus (sequence 2)

   

rAfum

5′- CATCTTCTTCGGCGGTG -3′

Reverse primer for loop sequence of A. fumigatus

   

rAnig

5′- CGTCTTCCTGGGCTGTTC -3′

Reverse primer for loop sequence of A. niger

   

rAter

5′- GGATTCCGACTATCATCCGTATAG -3′

Reverse primer for loop sequence of A. terreus

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