Fig. 2From: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzymeGenome-specific amplification of loop-encoding DNA sequences. a. A Vibrio-specific forward primer (fV) was used in combination with species-specific reverse primers rValg, rVpar and rVvul to selectively amplify the loop-encoding regions. 1.0Â ng genomic DNA of V. alginolyticus (Va), V. parahaemolyticus (Vp) and V. vulnificus (Vv) was used as template. The respective PCR products have a length of 204, 245 and 231Â bp, respectively. b. The corresponding gene region from 3.0Â ng genomic DNA from A. fumigatus, A. niger and A. terreus was amplified with appropriate primers (fA with rAfum, rAnig or rAter), leading to PCR products of 333 (A. fumigatus, A. niger) and 301Â bp (A. terreus). Reaction products were separated on a 2Â % agarose gel and stained with ethidium bromide. N, PCR negative control. M, 50Â bp DNA ladder (NEB)Back to article page