Fig. 2
From: Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis
Genetic analysis of the flagellin-encoding genes of L. agilis BKN88. a Partial gene map of the L. agilis motility operon. PCR amplification primers and the HindIII site are shown. Filled arrows represent putative glycosyltransferases. b Validation of flagellin-specific primers. Partial fragments of fliC1 and fliC2 were amplified by PCR using the specific primers and digested with HindIII. c Transcription of fliC1/fliC2 was detected by RT-PCR. PCR without reverse transcription was also performed to detect contaminated DNA