The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri

Table 2 Strains and plasmids used in this study

Strains and plasmids	Characteristics	Reference or source
Strains
Escherichia coli		[50]
DH5α	F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 real1 ∆ (argE-lacZYA) 169 Ø lazA ∆ M15	Promega
Xanthomonas citri subsp. citri
306	Syn. X. axonopodis pv. citri strain 306; wild-type, Rf^r, Ap^r	[25, 51]
∆hrpB (hrpB-)	hrpB (XAC0293): pNPTS138 derivative	This study
∆hrpB-p53hrpB (hrpB+)	hrpB (XAC0293) contained in pUFR053, Gm^r	This study
Plasmids
pGEM T-Easy	PCR cloning and sequencing vector, lacZ, Ap^r	Promega
pNPTS138	Km^r, sacB, lacZα⁺	M. R. Alley, unpublished
pUFR053	IncW Mob⁺ mob (P) lacZα⁺ Par+, Cm^r, Gm^r, Km^r, shuttle vector	[52]
pNPTS_hrpB	hrpB (XAC0293) gene cloning on pNPTS138	This study
p53_hrpB	hrpB (XAC0293) gene cloning on pUFR053	This study

Ap^r, Cm^r, Gm^r, Km^r, and Rf^r indicate resistance to ampicillin, chloromycetin, gentamicin, kanamycin and rifamycin, respectively

ISSN: 1471-2180