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Table 2 Oligonucletide sequences for PCR based amplification and sequencing

From: Relation between tetR and tetA expression in tetracycline resistant Escherichia coli

Primer

Sequence

Application

TetA-ybeM

for: 5’-ATGCTGGTGGCACTTCAGGCAGGAAACATCGTCGCCCGTTCAATCGTCACCCTTTCTCGG -3’

Recombination

 

rev: 5’-AGGCGGCAGGAAGTACCAGGATTTCAGCTCCCTGTCTATGTGCAACGGGAATTTGAAG-3’

 

TetA/ybeM

for: 5’-GAAGCATTGCTGGCGCGCGATG-3’

Proof of insertion

 

rev: 5’-TTCTCCAAAGCCCGCGACGCAG-

 

TetA

for: 5’-AAGAATCCGCGCGTTCAATCG-3’

Sequencing

 

rev: 5’-GCCCGGCACCGGCATAAT-3’

 

TetR

for: 5’-TCGGATCCTCAATCGTCACCCTTT-3’

Sequencing

 

for: 5’-CGCATCCATGCCGGCACG-3’

 

NusG

for: 5’-GTCCGTTCGCAGACTTTAAC-3’

qPCR

 

rev: 5’-GCTTTCTCAACCTGACTGAAG-3’

 

GapA

for: 5’-ACTGACTGGTATGGCGTTCC-3’

qPCR

 

rev: 5’-GTTGCAGCTTTTTCCAGACG-3’

 

TetA-qPCR

for: 5’-CGGTCTTCTTCATCATGCAAC -3’

qPCR

 

rev: 5’-GTCCCAGTGAAAGCGATCC-3’

 

TetR-qPCR

for: 5’-CCGAATGCGTATGATTCTCC-3’

qPCR

 

rev: 5’-CGCTTTACTGGCACTTCAGC-3’