Strain or Plasmid | Relevant Characteristic(s) | Reference or Source |
---|---|---|
Bacterial Strains | Â | Â |
O. tritici 5bvl1 | Type strain; Ampr; Cr(VI)r | Â |
chrC mutant | Single mutant of 5bvl1; chrC mutated | This study |
chrF mutant | Single mutant of 5bvl1; chrF mutated | This study |
chrC/chrF mutant | Double mutant of 5bvl1; chrC and chrF mutated | This study |
E. coli S17-1 | Conjugation donor strain | 22 |
E. coli 21(DE3) | F− ompT hsdS (rB -, mB −) gal dcm lacY1(DE3) | Novagen |
Plasmids | Â | Â |
pJQ200SK | Suicide vector; sacB; Gmr | ATCC |
pET30a | Kmr, expression plasmid | Novagen |
petChrC | pET30a for overproduction of ChrC with an C-terminal hexahistidine tag | This study |
petChrF | pET30a for overproduction of ChrF with an C-terminal hexahistidine tag | This study |
pchrC | pJQ200SK derivative carrying the upstream and downstream regions of chrC gene | This study |
Primers | Â | Â |
NdechrCf | ATACATATGTCCTTCGACATTAAACCGC | This study |
SalchrCr | TCCGTCGACTGAGAGTCCTCCTGAGGGTTG | This study |
NdechrFf | CGACATATGAAATGGATTACCCGCGAAC | This study |
SalchrFr | CGAGTCGACCATCTTCGGCGGCCAGTTATG | This study |
chrCupf | GCTGGATCCTCTGGCTTGCTGCGGGTTGCC | This study |
chrCupr | GCTCTGCAGTCACAAGATCAAGGTCTTCAA | This study |
chrCdownf | GCTCTGCAGATGAAATGGATTACCCGCGAA | This study |
chrCdownr | CGACTCGAGTCACATCTTCGGCGGCCAGTT | This study |
chrFupf | GCTGGATCCAACGGCGTGTTGCCCGTAGGC | This study |
chrFupr | GCTCTGCAGTCATGAGAGTCCTCCTGAGGG | This study |
chrF’f | GCTCTGCAGCCATGACGGTGAGCTTTGCAG | This study |