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Table 1 Bacterial strains, plasmids and primers used in this work

From: Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromate

Strain or Plasmid

Relevant Characteristic(s)

Reference or Source

Bacterial Strains

  

O. tritici 5bvl1

Type strain; Ampr; Cr(VI)r

 

chrC mutant

Single mutant of 5bvl1; chrC mutated

This study

chrF mutant

Single mutant of 5bvl1; chrF mutated

This study

chrC/chrF mutant

Double mutant of 5bvl1; chrC and chrF mutated

This study

E. coli S17-1

Conjugation donor strain

22

E. coli 21(DE3)

F− ompT hsdS (rB -, mB −) gal dcm lacY1(DE3)

Novagen

Plasmids

  

pJQ200SK

Suicide vector; sacB; Gmr

ATCC

pET30a

Kmr, expression plasmid

Novagen

petChrC

pET30a for overproduction of ChrC with an C-terminal hexahistidine tag

This study

petChrF

pET30a for overproduction of ChrF with an C-terminal hexahistidine tag

This study

pchrC

pJQ200SK derivative carrying the upstream and downstream regions of chrC gene

This study

Primers

  

NdechrCf

ATACATATGTCCTTCGACATTAAACCGC

This study

SalchrCr

TCCGTCGACTGAGAGTCCTCCTGAGGGTTG

This study

NdechrFf

CGACATATGAAATGGATTACCCGCGAAC

This study

SalchrFr

CGAGTCGACCATCTTCGGCGGCCAGTTATG

This study

chrCupf

GCTGGATCCTCTGGCTTGCTGCGGGTTGCC

This study

chrCupr

GCTCTGCAGTCACAAGATCAAGGTCTTCAA

This study

chrCdownf

GCTCTGCAGATGAAATGGATTACCCGCGAA

This study

chrCdownr

CGACTCGAGTCACATCTTCGGCGGCCAGTT

This study

chrFupf

GCTGGATCCAACGGCGTGTTGCCCGTAGGC

This study

chrFupr

GCTCTGCAGTCATGAGAGTCCTCCTGAGGG

This study

chrF’f

GCTCTGCAGCCATGACGGTGAGCTTTGCAG

This study

  1. Enzyme restriction site in primer sequences is shown in bold