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Table 1 Number of isolated Planktothrix rubescens filaments, percentage of PCR-positive filaments, and percentage of mutations

From: Emergence of nontoxic mutants as revealed by single filament analysis in bloom-forming cyanobacteria of the genus Planktothrix

 

Filament number

Positive Entry PCR (%)

Characteristics of filaments

Mutations within the mcy gene cluster (%)

Average (min, max) length (μm)

Average (min, max) cell numbera

MC synthesis not inactivated

Inactivation of MC synthesis

mcyTD insertion

mcyEG insertion

Short mcyA variant

mcyD insertion

mcyA insertion

mcyD deletion

mcyHA deletion

Mondsee (AT)

 Mar 2012

105

95.2

1356 (484, 2574)

435 (155, 825)

5.7

3.4

81.1

0

0

5.2

0

 Jun 2012

108

92.6

1251 (506, 2222)

401 (162, 712)

2.0

2.0

67.7

2.0

1.0

7.2

3.0

 Sep 2012

102

98

1653 (990, 2618)

530 (317, 839)

27.0

1.0

44.8

0

0

19.4

7.3

 Apr 2013

107

93.4

1392 (748, 2354)

446 (240, 754)

13.1

0

53.3

1.0

0

18.0

7.1

Other lakes

 Wörthersee (AT)

136

73.5

2200 (858, 6600)

705 (275, 2115)

18.4

36.0

67.3

1.0

4.0

5.0

3.1

 Zürichsee (CH)

121

82.6

1032 (440, 1738)

331 (141, 557)

25.3

9.0

45.0

0

0

1.0

1.0

 Hallwilersee (CH)

120

83.3

1555 (704, 2772)

498 (226, 888)

42.6

5.3

82.4

0

0

0

0

 Ammersee (DE)

115

87

1444 (638, 2420)

463 (204, 775)

37.6

4.2

60.6

0

0

0

0

 Total

914

87.5

1479 (440, 6600)

474 (141, 2115)

20.6

7.5

59

0.5

0.6

6.9

2.6

  1. aestimated from linear regression curve (Additional file 1)