No nucleotide | + ATP | + ADP | + ATPγS | |
---|---|---|---|---|
Single-stranded DNA | ||||
HpMutS2 | 24.82 ± 1.15 | 32.08 ± 5.32 | 28.11 ± 5.15 | 28.5 ± 3.68 |
HpMutS2-G338R | 40.19 ± 8.73 | 40.45 ± 9.52 | 38.87 ± 8.79 | ND |
HpMutS2-E413A | 6.50 ± 0.55 | 7.23 ± 0.65 | 6.90 ± 1.4 | ND |
HpMutS2ΔSmr-D30A | 2.12 ± 1.67 | ND | ND | ND |
Holliday junction | ||||
HpMutS2 | 26.49 ± 3.95 | 37.77 ± 8.59 | 18.85 ± 1.9 | 17.44 ± 0.84 |
HpMutS2-G338R | 41.59 ± 9.53 | 43.7 ± 11.42 | 41.6 ± 10.27 | ND |
HpMutS2-E413A | 4.92 ± 1.25 | 1.27 ± 1.01 | 6.77 ± 3.53 | ND |
HpMutS2ΔSmr-D30A | 3.34 ± 1.67 | ND | ND | ND |