Fig. 4From: Characterization of the murine macrophage response to infection with virulent and avirulent Burkholderia speciesQuantitation of Bp induced MNGC formation. RAW264.7 macrophages were infected with indicated Bp strain at a MOI of 30. Two hours or ten hours post infection cells were fixed and stained with antibodies that detect the bacteria or the host actin tails on bacterial surface. Image acquisition and analysis were performed as described in Fig. 2. a) Violin plots representing the quantitation of cellular attributes of the cluster population as measured by MNGC image analyses procedure. b) Representative confocal images of MNGC and actin tail formations. Nuclei are pseudocolored blue (hoechst dye), actin pseudocolored red (Phalloidin) and bacteria pseudocolored green (antibody). Scale bar - 50 μm. Data shown is representative of six replicates per plate, three plates per day and performed on three independent days. White arrows indicate MNGCsBack to article page