Fig. 5
EcN culture supernatants impair EPEC-mediated effects on epithelial cell barrier. Caco-2 monolayers grown on Transwell filters were incubated with EPEC alone (MOI 50) or with EPEC (MOI 50) in the presence of EcN cell-free culture supernatant (200 μg total protein). Incubation of Caco-2 cells with EcN supernatants (ECSN) were performed in parallel as a control. a TER values before (black bars) and after 2 hours incubation (white bars) were presented. b Paracellular passage of [3H]-D-mannitol from apical to basolateral compartments of Caco-2 monolayers after 2-h incubation. Data are expressed as the percentage of tracer recovery relative to the total tracer used (* P < 0.05;)