Fig. 4
Effect of EcN Sat on the paracellular permeability to 3H-mannitol in Caco-2 cell monolayers. Paracellular passage of [3H]-D-mannitol from apical to basolateral compartments of Caco-2 monolayers after 3-h incubation with suspensions of the indicated bacteria (MOI 100) (a) or with the corresponding concentrated culture supernatants (200 μg) (b). Equivalent amounts of recombinant Sat were assessed by Western blot. The last bar of each panel corresponds to cells incubated with the recombinant HB101(pSat) samples in the presence of EcN culture supernatant (indicated as EcNSN added). Data are presented as the percentage of tracer recovery relative to the total tracer used. Values were corrected subtracting the recovery of control cells incubated with the medium. Asterisks indicate values significantly different from that of EcNsat::cm (* P < 0.05; ** < 0.01)