Fig. 1

Western blot analysis of Sat secretion by EcN grown in LB. Several samples collected from an LB culture of EcN were processed and analysed with anti-Sat antibodies or with anti-LacZ antibodies as a control of cytosolic contamination: lane 1, TCA-precipitated cell-free supernatant before isolation of vesicles (total secreted proteins); lane 2, TCA-precipitated cell-free supernatant after removal of vesicles (soluble secreted proteins); lane 3, isolated OMVs; lane 4, cell extract (5 μg). IPTG (5 mM) was added to the culture medium to induce lacZ expression