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Table 3 Determination of free thiol groups in protein fusions

From: Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity

 

MalE-524Nter

MalE-524Nter, zinc

MalE-526Nter

MalE-526Nter, zinc

Thiol (μM)

17 ± 3

17 ± 2

8 ± 1

5 ± 0.5

Thiol/protein

0.85 ± 0.15

0.85 ± 0.1

0.4 ± 0.05

0.25 ± 0.02

  1. 20 μM of protein (equivalent to 80 μM of free thiol groups) was used for thiol group determination by DTNB assays
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BMC Microbiology

ISSN: 1471-2180

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