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Table 2 Llmg_0524 and Llmg_0526 are zinc metalloproteins

From: Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity

Protein

Ratio [Metal]/[protein]

Ratio [Zinc(PAR)2]/[protein]

 

Mg

Fe

Zn

 

MalE-0524Nter

0.21 ± 0.05

0.07 ± 0.01

0.45 ± 0.02

0.5 ± 0.07

MalE-0526 Nter

0.26 ± 0.02

0.54 ± 0.05

0.79 ± 0.05

0.8 ± 0.10

MalE-lacZα

0.58 ± 0.1

0

0.04 ± 0.01

ND

+ zinc

    

MalE-0524 Nter

0.20 ± 0.07

0.02 ± 0.01

0.42 ± 0.08

0.4 ± 0.03

MalE-0526 Nter

0.30 ± 0.11

0.03 ± 0.01

0.65 ± 0.14

0.8 ± 0.05

MalE-lacZα

0.17 ± 0.04

0

0.01 ± 0.01

ND

  1. All Buffers were treated with Chelex. Values are the mean of three independent protein purifications. + zinc, cultures were supplemented with 0.1 mM of zinc salt. MalE-lacZα (from plasmid, pMal c4x) is used as a control, ND: not determined
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BMC Microbiology

ISSN: 1471-2180

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