Fig. 4

Detection and quantitative estimation of 2-keto-3-deoxyoctonate (KDO) in pathogenic and intermediately pathogenic Leptospira species. LPS extracted from the pathogenic species Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130, and the intermediately pathogenic species Leptospira licerasiae serovar Varillal strain VAR010 was subjected to KDO analysis using reverse phase HPLC with a C18 column after tagging with a fluorophore 4, 5 methylenedioxy-1, 2-phenylenediamine dihydrochloride (DMB). a Copenhageni aqueous layer LPS; (b) VAR010 aqueous layer LPS; (c) Copenhageni phenol layer LPS; (d) VAR010 phenol layer LPS