Table 2 Results of specificity tests using plc- and cpe-targeted primer sets

Clostridium perfringens

ATCC 13124^T

+

–

Clostridium perfringens

ATCC 9856

+

–

Clostridium perfringens

ATCC 3624

+

–

Clostridium perfringens

ATCC 3626

+

–

Clostridium perfringens

ATCC 12917

+

+

Clostridium perfringens

ATCC 14809

+

+

Clostridium perfringens

ATCC 27324

+

–

Clostridium perfringens

C052-1

+

+

Clostridium ramosum

JCM 1298^T

–

–

Clostridium innocuum

DSM 1286^T

–

–

Clostridium cocleatum

JCM 1397^T

–

–

Clostridium symbiosum

JCM 1297^T

–

–

Clostridium ghonii

JCM 1400^T

–

–

Clostridium aminovalericum

JCM 11016^T

–

–

Clostridium indolis

JCM 1380^T

–

–

Clostridium paraputrificum

JCM 1293^T

–

–

Clostridium butylicum

JCM 1391^T

–

–

Clostridium difficile

DSM 1296^T

–

–

Clostridium sordellii

JCM 3814^T

–

–

Clostridium glycolicum

JCM 1401^T

–

–

Clostridium lituseburense

JCM 1404^T

–

–

Clostridium bifermentans

JCM 1386^T

–

–

Blautia producta

JCM 1471^T

–

–

Faecalibacterium prausnitzii

ATCC 27768^T

–

–

Bacteroides vulgatus

ATCC 8482^T

–

–

Collinsella aerofaciens

DSM 3979^T

–

–

Prevotella melaninogenica

ATCC 25845^T

–

–

Bifidobacterium longum

ATCC 15707^T

–

–

Clostridium innocuum

DSM 1286^T

–

–

Fusobacterium russii

ATCC 25533^T

–

–