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Fig. 6 | BMC Microbiology

Fig. 6

From: A GntR family transcription factor positively regulates mycobacterial isoniazid resistance by controlling the expression of a putative permease

Fig. 6

Determinations of growth curves of M. smegmatis strains. Mycobacterial strains were grown in 7H9 medium in the presence of 10 μg/ml isoniazid, and growth curves were determined. (a) Left: the wild-type (WT/pMV261) and Ms0535-overexpressing (WT/pMV261-Ms0535) strains; Right: the WT (WT/pMV261) and Ms0534-overexpressing (WT/pMV261-Ms0534) strains. To avoid potential side effects, the empty pMV261vector was included in the WT strain. (b) Left: the WT (WT/pMindD), Ms0535 deletion (ΔMs0535/pMindD), and ΔMs0535 complemented (ΔMs0535/pMindD-Ms0535) strains; Right: the WT (WT/pMindD), Ms0534 deletion (ΔMs0534/pMindD), and ΔMs0534 complemented (ΔMs0534/pMindD-Ms0534) strains. To avoid potential side effects, the empty pMindD vector was included in the WT and Ms0534/Ms0535 deletion strains. (c) Left: the Ms0535 deletion (ΔMs0535/pMV261) strain and the ΔMs0535 strain complemented with Ms0534 (ΔMs0535/pMV261-Ms0534); Right: the Ms0534 deletion strain (ΔMs0534/pMV261) and the ΔMs0534 strain complemented with Ms0535 (ΔMs0534/pMV261-Ms0535). To avoid potential side effects, the empty pMV261 vector was included in the Ms0534/Ms0535 deleted strains. Error bars represent the standard deviation of three biological replicates

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BMC Microbiology

ISSN: 1471-2180

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