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Fig. 4 | BMC Microbiology

Fig. 4

From: A GntR family transcription factor positively regulates mycobacterial isoniazid resistance by controlling the expression of a putative permease

Fig. 4

Assays for Ms0534-Ms0535 co-transcription by reverse transcription-PCR. (a) The schematic operon structure of Ms0534-Ms0535. Primers designed for the assays are indicated by black arrows; F1 primers are complementary to the sequences of the two adjacent genes. (b) Reverse transcription-PCR assays for Ms0534-Ms0535 co-transcription. In this assay, the resulting cDNA was transcribed by the F3 reverse primer. For the F1 fragment, mRNA (DNA-free) was used as a negative control (lane 1). In lane 2, a 189-bp reverse transcription-PCR product indicates the co-transcription of Ms0534-Ms0535. The 146-bp F2 and 100-bp F3 extension products are shown in lanes 2 and 3. The negative result in lane 5 reveals that the F4 fragment cannot be amplified from the cDNA template. The PCR procedure was as follows: the reactions underwent 35 cycles of denaturation at 95 °C for 30 s, annealing at 60 °C for 30 s, and extension at 72 °C for 30 s

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