Fig. 1

The 3-ketoacyl-ACP reductase (KAR) reaction, alignment of R. solanacearum FabG1 and FabG2 with E. coli FabG and organization of the R. solanacearum fatty acid biosynthesis gene clusters. Panel a, The KAR reaction. Panel b, Alignments of R. solanacearum FabG1 and FabG2 with E. coli FabG. Rs and Ec denote R. solanacearum and E. coli, respectively. The cofactor binding sequence (Gly motif [GlyXXXGlyXGly]) is boxed. The two arginine residues thought to bind the ACP moiety are highlighted by arrows and the catalytic triad residues are highlighted by asterisks. The alignment was done with Clustal W based on identical residues. Panel c, Organization of the R. solanacearum fatty acid biosynthesis gene clusters. The thick arrows indicate the relative sizes of the genes. The numbers above the arrows indicate the gene designations in the R. solanacearum of Comprehensive Microbial Resource (CMR) database, and the gene names below the arrows indicate the E. coli genes that correspond to the open reading frames in the R. solanacearum cluster. HSP indicates hypothetical signal peptide protein. CHP indicates conserved hypothetical protein. KAS indicates putative 3-ketoacyl-acyl carrier protein synthase