Multilocus sequence typing of Streptococcus thermophilus from naturally fermented dairy foods in China and Mongolia

Table 2 Genes and primers used for Multilocus sequence typing

Gene	Amplicon size (bp)	Application size (bp)	Gene function	PCR primer	Sequences (5’-3’)	Temperature
carB	652	499	carBamoyl phosphate synthetase	carB_primerF	AAGGCTACAGTGTTGTTCT	51 °C
			carBamoyl phosphate synthetase	carB_primerR	GATAAGGTTTGCGTTGG
clpX	768	630	ATP-dependent Clp protease subunit X	clpX_primerF	GCAGGTTATGTGGGTGA	52 °C
			ATP-dependent Clp protease subunit X	clpX_primerR	TACTGGAGCAGCTTTCC
dnaA	669	522	chromosomal replication initiator protein dnaA	dnaA_primerF	CACCAGGAGCAACTTAT	49 °C
			chromosomal replication initiator protein dnaA	dnaA_primerR	CTTCAATCGGAATGAGA
murC	592	451	UDP-N-acetyl muramate-alanine ligase	murC_primerF	TTTGTTTACGGTGAGGA	51 °C
			UDP-N-acetyl muramate-alanine ligase	murC_primerR	CCACTTTGGCAGGTTTA
murE	721	592	UDP-N-acetylmuramyl tripeptide synthase	murE_primerF	AACACCCTCAAGACAAA	48 °C
			UDP-N-acetylmuramyl tripeptide synthase	murE_primerR	ACGAATACCTTAGCACC
pepN	737	615	lysyl-aminopeptidase, aminopeptidase N	pepN_primerF	CGCTGAAGAGGGCGATAC	58 °C
			lysyl-aminopeptidase, aminopeptidase N	pepN_primerR	CTAACCAACGGCGGAGC
pepX	664	524	X-prolyl dipeptidyl aminopeptidase	pepX_primerF	ACATCCCTGTTAGTCCTG	52 °C
			X-prolyl dipeptidyl aminopeptidase	pepX_primerR	TCTCCCTCCATCTTGTG
pyrG	736	624	CTP synthase	pyrG_primerF	CACTGAAGTTGGTGGGA	52 °C
			CTP synthase	pyrG_primerR	CATACCGAGGCAGACAC
recA	768	617	recombinase A	recA_primerF	AAAGAAGGTGGCATCGC	58 °C
				recA_primerR	ATCGTCCTCATCTAGCTCAAC
rpoB	786	661	RNA polymerase beta subunit	rpoB_primerF	CATTACACGCACTACGG	47 °C
				rpoB_primerR	GATAACAGCATCCTCGA

ISSN: 1471-2180