Fig. 4

H. pylori type IV secretion system is not required for complete PDK-1 dephosphorylation. a AGS cell lysates were separated by SDS-PAGE followed by Western blotting with antibodies to phospho Ser 241 PDK-1 (upper panel), total PDK-1 protein (middle panel), or Hsp27 (lower panel). Total PDK-1 protein ran as two distinct species, one at the expected molecular weight (arrow) and at a faster migrating band (*) that was likely proteolytically cleaved. b PDK-1 phosphorylation levels and total protein were quantified by normalizing to Hsp27 levels and the amount of phosphorylation or total protein expressed as a ratio of control protein, i.e. (phospho protein/Hsp27)/(total protein/Hsp27). Data are representative of at least 3 different experiments and presented as a percent of control and indicate the relative amount of phosphorylation or protein remaining in the system