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Fig. 2 | BMC Microbiology

Fig. 2

From: Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response

Fig. 2

Response of oxidative stress-associated genes and promoters. a Expression of katA, ahpC, sodB, and osmC from P. putida mt-2 in the presence of H2O2 (1.5 mM), paraquat (PQ, 100 μM), or without an oxidative stress inducer (control, Ctrl) as measured by RT-qPCR. Cells were sampled 30 min and 2 h after addition of the stress inducer. Data are mean values ± S.D. (n = 3) of mRNA per DNA of the gene in question. Asterics (*) indicate mean values of treated cultures that are significantly (P < 0.05) higher than mean values from Ctrl cultures. Note the different scale for the y-axis of the diagram of osmC expression. b Induction of pSEVA237M-based bioreporters in P. putida mt-2 cultured in the presence of H2O2 (1.5 mM) or PQ (100 μM) after 2–8 h of stress exposure. Data are mean values ± S.D. (n = 3) of average fluorescence intensity of cells treated with either H2O2 or PQ normalized to the average fluorescence intensity of Ctrl cells. Asterisks (*) indicate mean values from treated cultures that are significantly (P < 0.05) higher than mean values from Ctrl cultures. Note that values on the y-axis of the diagram of P katA and P ahpC are different from the values in the diagram of P sodB and P osmC

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