Target strain | Target gene | Primer sequence (5′ → 3′) | Amplicon size (bp) | PCR cycling condition | Reference |
---|---|---|---|---|---|
E. coli | uidA | F: AAA ACG GCA AGA AAA AGC AG | 147 | 5 min initial denaturation at 94 °C followed by 35 cycles of 95 °C for 30 s, 58 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 8 min | [90] |
R: ACG CGT GGT TAA CAG TCT TGC G | |||||
EPEC | eaeEae | F: TCA ATG CAG TTC CGT TAT CAG TT | 482 | 15 min initial denaturation at 95 °C followed by 35 cycles of 94 °C for 45 s, 55 °C for 45 s, 68 °C for 2 min and final extension at 72 °C for 5 min | [93] |
R: GTA AAG TCC GTT ACC CCA ACC TG | |||||
 | bfpBfp | F: GGA AGT CAA ATT CAT GGG GGT AT | 300 | 2 min initial denaturation at 94 °C followed by 40 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [93] |
R: GGA ATC AGA CGC AGA CTG GTA GT | |||||
ETEC | ltLt | F: GGC GAC AGA TTA TAC CGT GC | 450 | 2 min initial denaturation at 94 °C followed by 35 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [94] |
G: CGG TCT CTA TAT TCC CTG TT | |||||
EAEC | eaggEagg | F: AGA CTC TGG CGA AAG ACT GTA TC | 194 | 15 min initial denaturation at 95 °C followed by 35 cycles of 94 °C for 45 s, 55 °C for 45 s, 68 °C for 2 min and final extension at 72 °C for 5 min | [95] |
R: ATG GCT GTC TGT AAT AGA TGA GAA C | |||||
EIEC | ipaH | F: CTC GGC ACG TTT TAA TAG TCT GG | 933 | 2 min initial denaturation at 94 °C followed by 35 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [96] |
R: GTG GAG AGC TGA AGT TTC TCT GC | |||||
DAEC | daaE | F: GAA CGT TGG TTA ATG TGG GGT AA | 542 | 2 min initial denaturation at 94 °C followed by 40 cycles of 92 °C for 30 s min, 59 °C for 30 s, 72 °C for 30 s and final extension at 72 °C for 5 min | [96] |
R: TAT TCA CCG GTC GGT TAT CAG T | |||||
EHEC | stx1 | F: CAG TTA ATG TGG TGG CGA AGG | 384 | 15 min initial denaturation at 95 °C followed by 35 cycles of 94 °C for 45 s, 55 °C for 45 s, 68 °C for 2 min and final extension at 72 °C for 5 min | [72] |
R: CAC CAG ACA ATG TAA CCG CTG | |||||
 | stx2 | F: ATC CTA TTC CCG GGA GTT TAC G | 584 | 2 min initial denaturation at 94 °C followed by 35 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [72] |
R GCG TCA TCG TAT ACA CAG GAG C | |||||
NMEC | ibeA | F: TGG AAC CCC GCT CGT AAT ATA C | 342 | 2 min initial denaturation at 94 °C followed by 30 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [96] |
R: CTG CCT GTT CAA GCA TTG CA | |||||
UPEC | papC | F: GAC GGC TGT ACT GCA GGG TGT GGC G | 328 | 2 min initial denaturation at 94 °C followed by 30 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 1 min and final extension at 72 °C for 5 min | [97] |
R: ATA TCC TTT CTG CAG GGA TGC AAT A |