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Table 2 Enzymatic profile of B. hyodysenteriae type strain (B78T) and six strains of B. suanatina

From: Brachyspira suanatina sp. nov., an enteropathogenic intestinal spirochaete isolated from pigs and mallards: genomic and phenotypic characteristics

Strain

Brachyspira panel a

api-ZYM enzyme activitiesb

Haem

IS

Hipp

α-gal

β-glu

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

B78T

s

+

+

0

4

3

1

0

0

0

0

1

2

4

1

1

5

2

4

4

0

0

0

AN4859/03

s

+

+

0

2

1

1

0

0

0

0

0

0

2

1

0

5

0

2

4

0

0

0

AN1681:1/04

s

+

+

0

2

1

1

0

0

0

0

0

0

2

1

0

4

0

2

3

0

0

0

AN2384/04

s

+

+

0

2

1

1

0

0

0

0

0

0

1

2

0

5

0

2

4

0

0

0

Dk12570-2

s

+

+

0

3

1

1

0

0

0

0

0

0

2

1

0

5

0

2

3

0

0

0

AN3949:2/02

s

+

+

0

3

1

1

0

0

0

0

0

0

2

1

0

5

0

2

3

0

0

0

AN1418:2/01

s

+

+

0

2

1

1

0

0

0

0

0

0

3

1

0

5

0

3

4

0

0

0

  1. a Brachyspira panel according to Fellström et al., 1999. Results according to Råsbäck et al., 2007. Haem = intensity of β-haemolysis; IS = indole spot test; Hipp = hippurate cleavage capacity; α-galactosidase; β-glucosidase; s = strong reaction; + = positive reaction, − = negative reaction
  2. bAPI-ZYM test: 1 control; 2 alkaline phosphatase; 3 C4 esterase; 4 C8 esterase lipase; 5 C14 lipase; 6 leucine arylamidase; 7 valine arylamidase; 8 cystine arylamidase; 9 trypsin; 10 chymotrypsin; 11 acid phosphatase; 12 naphtolphosphohydrolase; 13 α-galactosidase; 14 β-galactosidase; 15 β-glucoronidase; 16 α-glucosidase; 17 β-glucosidase; 18 N-acetyl-β-glucosaminidase; 19 α-mannosidase; 20 α-fucosidase; values 0–5 assigned according to the manufacturer’s instruction 0 = negative reaction, 5 = maximum intensity