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Fig. 4 | BMC Microbiology

Fig. 4

From: Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species

Fig. 4

Multiplexed Luminex immunoassay for detecting potential bioterror agents, B. melitensis, B. anthracis, F. tularensis and Y. pestis. a Test samples contained B. melitensis 16 M (Bm, 5 × 105 cells/mL), B. anthracis PXO1+ (Ba, 5 × 105 cells/mL), F. tularensis 6223 (Ft, 5 × 105 cells/mL) and Y. pestis CO92 (Yp, 5 × 104 cells/mL) cells in PBS either alone or in combination. In (b), PBS and milk samples were spiked with all four bacterial species and used at a concentration of 2.5 × 106 cells/mL. MAbs 3D12, MTA1, T14 and YPF19, coupled to distinct magnetic beads, were used as capture antibodies and the biotinylated mAbs 10G1, MTD6, FB11 and YPF19 were used for detection. Reporter dye fluorescence intensities measured for each bead set are shown. Dashed lines indicate the limit of detection (LOD) defined as mean blank plus three times the standard deviation

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