Table 4 Bioinformatic analysis of identified and quantified proteins in the secretome of C. neoformans under Pka1-repressed and Pka1-induced conditions
GO categoriesa | Accession number | Protein name | Signal peptideb (position) | GPI anchorc (position) | Sample preparation |
|---|---|---|---|---|---|
Carbohydrate catabolic process | Â | Â | Â | ||
|  | CNAG_02189 | α-Amylase | Yes (21/22) | Yes (C-26) | EtOH, TCA/acetone |
GTP catabolic process | Â | Â | Â | ||
|  | CNAG_06125 | Translation elongation factor 1 α | No | No | EtOH, TCA/acetone |
| Â | CNAG_06840 | Translation elongation factor 2 | No | No | TCA/acetone |
Carbohydrate metabolic process | Â | Â | Â | ||
| Â | CNAG_00799 | Cellulase | Yes (22/23) | No | TCA/acetone |
| Â | CNAG_01239 | Chitin deacetylase | Yes (18/19) | Yes (C-28) | EtOH, TCA/acetone |
|  | CNAG_02860 | Endo-1,3(4)-β-glucanase | Yes (22/23) | No | EtOH |
| Â | CNAG_04245 | Chitinase | Yes (21/22) | No | EtOH, TCA/acetone |
| Â | CNAG_05799 | Chitin deacetylase | Yes (18/19) | Yes (C-28) | EtOH |
| Â | CNAG_06291 | Deacetylase | Yes (19/20) | No | EtOH, TCA/acetone |
| Â | CNAG_06313 | Phosphoglucomutase | No | No | TCA/acetone |
|  | CNAG_06501 | 1,3-β-glucanosyltransferase | Yes (20/21) | Yes (C-6) | EtOH, TCA/acetone |
Cellular carbohydrate metabolic process | Â | Â | Â | ||
| Â | CNAG_03225 | Malate dehydrogenase | No | No | EtOH, TCA/acetone |
Trehalose metabolic process | Â | Â | Â | ||
| Â | CNAG_03525 | Trehalase | Yes (18/19) | No | TCA/acetone |
Glyoxylate metabolic process | Â | Â | Â | ||
| Â | CNAG_01137 | Aconitase | No | No | TCA/acetone |
Pentose-phosphate pathway | Â | Â | Â | ||
| Â | CNAG_01984 | Transaldolase | No | No | TCA/acetone |
| Â | CNAG_07561 | Phosphogluconate dehydrogenase | No | No | EtOH |
Protein ubiquitination | Â | Â | Â | ||
| Â | CNAG_01920 | Polyubiquitin | No | No | EtOH |
ATP hydrolysis coupled proton transport | Â | Â | Â | ||
|  | CNAG_05750 | ATPase α subunit | No | No | EtOH, TCA/acetone |
|  | CNAG_05918 | F0F1 ATP synthase subunit β | No | No | EtOH |
Transmembrane transport | Â | Â | Â | ||
| Â | CNAG_02974 | Voltage-dependent ion-selective channel | No | No | TCA/acetone |
| Â | CNAG_06101 | Eukaryotic ADP/ATP carrier | No | Yes (C-25) | EtOH, TCA/acetone |
Methionine biosynthetic process | Â | Â | Â | ||
| Â | CNAG_01890 | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase | No | No | EtOH |
Dephosphorylation | Â | Â | Â | ||
| Â | CNAG_02944 | Acid phosphatase | Yes (16/17) | No | EtOH, TCA/acetone |
Glycolysis | Â | Â | Â | ||
| Â | CNAG_03072 | Phosphopyruvate hydratase | No | No | EtOH, TCA/acetone |
| Â | CNAG_06699 | Glyceraldehyde-3-phosphate dehydrogenase | No | No | TCA/acetone |
Oxidation-reduction process | Â | Â | Â | ||
| Â | CNAG_01019 | Cu/Zn superoxide dismutase | No | No | EtOH |
| Â | CNAG_03465 | Laccase | Yes (20/21) | No | EtOH, TCA/acetone |
Proteolysis | Â | Â | Â | ||
| Â | CNAG_00581 | Endopeptidase | Yes (19/20) | No | TCA/acetone |
| Â | CNAG_00919 | Carboxypeptidase D | Yes (21/22) | No | EtOH, TCA/acetone |
Response to stress | Â | Â | Â | ||
| Â | CNAG_00334 | Heat shock protein | No | No | TCA/acetone |
| Â | CNAG_01727 | Hsc70-4 | No | No | EtOH |
| Â | CNAG_01750 | Chaperone | No | No | EtOH, TCA/acetone |
| Â | CNAG_06150 | Heat-shock protein 90 | No | No | TCA/acetone |
Translation | Â | Â | Â | ||
| Â | CNAG_04021 | 60S ribosomal protein L26 | No | No | TCA/acetone |
| Â | CNAG_04114 | 40S ribosomal protein S0 | No | No | TCA/acetone |
| Â | CNAG_06095 | Ribosomal protein L13 | No | No | EtOH |
| Â | CNAG_06605 | Ribosomal protein S2 | No | No | TCA/acetone |
Regulation of transcription | Â | Â | Â | ||
| Â | CNAG_00483 | Actin | No | No | TCA/acetone |
Nucleosome assembly | Â | Â | Â | ||
| Â | CNAG_06746 | Histone h2b | No | No | TCA/acetone |
Unknown/Unclassified | Â | Â | Â | ||
| Â | CNAG_00407 | Glyoxal oxidase | Yes (16/17) | Yes (C-27) | EtOH, TCA/acetone |
| Â | CNAG_00776 | Immunoreactive mannoprotein MP88 | Yes (22/23) | Yes (C-30) | TCA/acetone |
| Â | CNAG_01653 | Cytokine-inducing glycoprotein | Yes (19/20) | No | EtOH |
| Â | CNAG_02030 | Glyoxal oxidase | Yes (21/22) | Yes (C-32) | EtOH, TCA/acetone |
|  | CNAG_02850 | Glucan endo-1,3-α-glucosidase agn1 | Yes (21/22) | Yes (C-26) | TCA/acetone |
| Â | CNAG_02864 | Predicted protein | Yes (16/17) | No | TCA/acetone |
| Â | CNAG_02943 | Cytoplasmic protein | No | No | TCA/acetone |
| Â | CNAG_04291 | Glycosyl-hydrolase | Yes (17/18) | Yes (C-31) | EtOH |
| Â | CNAG_04753 | Lactonohydrolase | No | No | EtOH, TCA/acetone |
| Â | CNAG_06267 | Rds1 protein | Yes (20/21) | Yes | EtOH, TCA/acetone |
Hypothetical | Â | Â | Â | ||
| Â | CNAG_00586 | Conserved hypothetical protein | Yes (23/24) | No | EtOH, TCA/acetone |
| Â | CNAG_00587 | Conserved hypothetical protein | Yes (19/20) | No | TCA/acetone |
| Â | CNAG_00588 | Conserved hypothetical protein | Yes (16/17) | No | EtOH |
| Â | CNAG_01047 | Conserved hypothetical protein | Yes (19/20) | No | EtOH, TCA/acetone |
| Â | CNAG_01562 | Conserved hypothetical protein | Yes (18/19) | No | EtOH |
| Â | CNAG_03007 | Conserved hypothetical protein | No | No | EtOH |
| Â | CNAG_03223 | Conserved hypothetical protein | Yes (19/20) | Yes (C-18) | EtOH |
| Â | CNAG_03492 | Conserved hypothetical protein | Yes (19/20) | No | TCA/acetone |
| Â | CNAG_05312 | Conserved hypothetical protein | Yes (19/20) | Yes (C-19) | EtOH, TCA/acetone |
| Â | CNAG_05595 | Conserved hypothetical protein | Yes (18/19) | Yes (C-29) | TCA/acetone |
| Â | CNAG_05893 | Conserved hypothetical protein | Yes (16/17) | Yes (C-13) | EtOH, TCA/acetone |
| Â | CNAG_06109 | Conserved hypothetical protein | No | No | EtOH |