Table 2 Candida albicans proteins with the previously confirmed binding affinity to human fibronectin, vitronectin and laminin
Abbreviation | Protein name | Method used for protein identification and confirmation of binding to ECMPs | Reference |
|---|---|---|---|
Fibronectin-binding proteins | |||
Als1 | Agglutinin-like sequence protein 1 | expression in Saccharomyces cerevisiae followed by binding to magnetic beads coated with fibronectin | [38] |
expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin | [16] | ||
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements | [39] | ||
Als3 | Agglutinin-like sequence protein 3 | expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin | [16] |
expression in S. cerevisiae followed by microtiter plate-based assay | [40] | ||
Als5 | Agglutinin-like sequence protein 5 | expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin | [16] |
binding of protein fragments—Als5 1–431 and Als5 1-664—to fibronectin-coated microtiter plate wells | [41] | ||
Hwp1 | Hyphal cell wall protein 1 | expression in S. cerevisiae followed by microtiter plate-based assay | [40] |
Tdh3 | Glyceraldedyhde-3-phosphate dehydrogenase | immunoaffinity purification of Tdh3 followed by ligand Western Blot assay | [18] |
Adh1 | Alcohol dehydrogenase | screening of C. albicans cDNA library with polyclonal antibodies against receptor for fibronectin | [17] |
Vitronectin-binding proteins | |||
Adh1 | Alcohol dehydrogenase | screening of C. albicans cDNA library with polyclonal antibodies against receptor for vitronectin | [17] |
Gpm1 | Phosphoglycerate mutase | enzyme-linked ligand sorbent assay (ELISA) with microplate-immobilized recombinant Gpm1 | [45] |
Laminin-binding proteins | |||
Als1 | Agglutinin-like sequence protein 1 | expression in S. cerevisiae followed by binding to magnetic beads coated with laminin | [38] |
expression in S. cerevisiae followed by binding to microplate-immobilized laminin | [16] | ||
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements | [39] | ||
Als3 | Agglutinin-like sequence protein 3 | expression in S. cerevisiae followed by binding to microplate-immobilized laminin | [16] |
Als5 | Agglutinin-like sequence protein 5 | expression in S. cerevisiae followed by binding to microplate-immobilized laminin | [16] |
Tdh3 | Glyceraldedyhde-3-phosphate dehydrogenase | immunoaffinity purification of Tdh3 followed by ligand Western Blot assay | [18] |