Table 1 PCR primers for real-time RT-PCR
Gene | Primer direction | Sequence(5’-3’) | Amplicon size (bp) |
|---|---|---|---|
lasI | Forward | GGCTGGGACGTTAGTGTCAT | 104 |
Reverse | AAAACCTGGGCTTCAGGAGT | ||
lasR | Forward | ACGCTCAAGTGGAAAATTGG | 111 |
Reverse | TCGTAGTCCTGGCTGTCCTT | ||
rhlI | Forward | AAGGACGTCTTCGCCTACCT | 130 |
Reverse | GCAGGCTGGACCAGAATATC | ||
rhlR | Forward | CATCCGATGCTGATGTCCAACC | 101 |
Reverse | ATGATGGCGATTTCCCCGGAAC | ||
lasA | Forward | GCGCGACAAGAGCGAATAC | 94 |
Reverse | CGGCCCGGATTGCAT | ||
lasB | Forward | AGACCGAGAATGACAAAGTGGAA | 81 |
Reverse | GGTAGGAGACGTTGTAGACCAGTTG | ||
phzH | Forward | TGCGCGAGT TCAGCCACCTG | 214 |
Reverse | TCCGGGACATAGTCGGCGCA | ||
rhlA | Forward | TGGCCGAACATTTCAACGT | 107 |
Reverse | GATTTCCACCTCGTCGTCCTT | ||
rpsL | Forward | GCAACTATCAACCAGCTGGTG | 231 |
Reverse | GCTGTGCTCTTGCAGGTTGTG |