Fig. 2

Phytoplasma internalization assay: immunolocalization of chrysanthemum yellows phytoplasma (CYP) in vibratome sections of salivary glands dissected from healthy Euscelidius variegatus. In a-f, salivary glands were incubated for 4 h with an extract of CYP infected Macrosteles quadripunctulatus: CYPs are visible as isolated cells (arrowheads in a and b) and grouped within vesicles (c-f). In g and h salivary glands were incubated for 4 h with an extract of healthy Macrosteles quadripunctulatus (negative controls). In i-n, salivary glands were incubated for 4 h with CYP suspension added with A416 antibody: CYPs are visible only as isolated cells (arrowheads). a, c, e, g, i, m show fluorescence images; b, d, f, h, l, n are the corresponding merged light and fluorescence images. Bars represent 20 μm