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Table 2 Stability of plasmids and mini-Tn7 insertions in different strains

From: Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria

Strain

Free-living stabilitya

Stability in symbiosisb

Ret pJB3Tc19

8,6 %

98 %

Ret pJBpleD*

0.6 %

0 %

Ret Tn7Km

100 %

100 %

Ret Tn7pleD*Km

100 %

90 %

Ret Tn7Tc

100 %

n.d.

Ret Tn7pleD*Tc

100 %

n.d.

Rle pJB3Tc19

73,5 %

86 %

Rle pJBpleD*

67,1 %

70 %

Rle Tn7Km

100 %

100 %

RleTn7pleD*Km

100 %

100 %

Rle Tn7Tc

100 %

n.d.

Rle Tn7pleD*Tc

n.d

n.d.

Sme pJB3Tc19

2,8 %

51 %

Sme pJBpleD*

3,1 %

22 %

Sme Tn7Km

100 %

100 %

Sme Tn7pleD*Km

100 %

91 %

Sme Tn7Tc

100 %

n.d.

Sme Tn7pleD*Tc

100 %

n.d.

Pto pJB3Tc19

100 %

n.d.

Pto pJBpleD*

76,8 %

n.d.

Pto Tn7Km

100 %

n.d.

Pto Tn7pleD*Km

100 %

n.d.

Pto Tn7Tc

100 %

n.d.

Pto Tn7pleD*Tc

100 %

n.d.

  1. aBacteria were grown in rich medium without antibiotics for at least 100 generations; the stability was determined as the ratio of CFU recovered on selective medium out of the total CFU obtained in nonselective medium
  2. bPercentage of nodules containing bacteria that kept antibiotic resistance (for more details see supporting information)
  3. n.d., not determined