Expression of calpain-like proteins and effects of calpain inhibitors on the growth rate of Angomonas deanei wild type and aposymbiotic strains

Table 2 Detection of cross-reactivity between calpain-like proteins from Angomonas deanei wild type and aposymbiotic strains and anti-calpain antibodies by flow cytometric analysis

Anti-calpain Antibody	Non-Permeabilized Parasites				Triton X-100-Permeabilized Parasites
Anti-calpain Antibody	Wild Type Strain		Aposymbiotic Strain		Wild Type Strain		Aposymbiotic Strain
	% FC^a	MFI^a	% FC	MFI	% FC	MFI	% FC	MFI
Anti-Dm-calpain	6.3 ± 0.4	4.1 ± 0.2	5.3 ± 0.5	4.6 ± 0.5	80.0 ± 3.9	44.4 ± 6.8**	81.1 ± 8.9	19.7 ± 3.2**
Anti-CAP5.5	8.2 ± 0.8	4.7 ± 0.3	6.0 ± 1.0	4.3 ± 0.5	22.1 ± 1.8*	10.1 ± 0.2	44.2 ± 9.9*	13.5 ± 0.6
Anti-CDPIIb	6.1 ± 0.9	2.8 ± 0.2	4.4 ± 1.2	2.4 ± 0.2	39.8 ± 4.0	16.9 ± 1.5	39.9 ± 5.4	18.6 ± 0.4

^a % FC, percentage of fluorescent cells; MFI, mean of fluorescence intensity. Symbols (*,**) denote significant different concerning either the % FC (*) or MFI (**) between wild type and aposymbiotic strains (P <0.05). Additionally, both the percentage of antibody-labeled cells and the MFI values for all tested antibodies were significantly different between non-permeabilized and Triton X-100-permeabilized parasite cells. Representative data of the analysis of 10,000 cells from 3 experiments are shown

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