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Fig. 1 | BMC Microbiology

Fig. 1

From: Expression of calpain-like proteins and effects of calpain inhibitors on the growth rate of Angomonas deanei wild type and aposymbiotic strains

Fig. 1

Detection of cross-reactivity between calpain-like proteins (CALPs) from Angomonas deanei wild type (w) and aposymbiotic (a) strains and anti-calpain antibodies by Western blotting. CALPs recognized in the whole cellular extract (upper panel) and in the conditioned supernatant (lower panel) by the anti-Dm-calpain, anti-CAP5.5 and anti-CDPIIb antibodies in each strain are shown. Anti-α-tubulin monoclonal antibody was used as a control for sample loading in the blots, revealing a protein band of 50 kDa in similar amount in both strains. The apparent molecular masses, expressed in kilodaltons, of each detected band are shown, and the densitometric analysis of the reactive bands is expressed as densitometric units (D.U.). The results represent means standard deviation of three independent experiments, and the asterisks denote statistic difference between wild type and aposymbiotic strains (P <0.05)

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