Fig. 9
Effect of reducing conditions on breakdown of haemalbumin by InpA at different pH. Haemalbumin (16 μM) with a 1:1 haem to protein ratio was incubated at 37 °C with InpA (100 μg ml−1) in the absence (a panels) and presence of 3 mM DTT (b panels). Samples and gels were run under non-reducing conditions on 10 % gels and stained for protein with CBB. Each gel lane had a nominal loading of 20 μg albumin